您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genetic architecture of protein expression and its regulation in the mouse brain

文献类型： 外文期刊

作者： Erickson, Alyssa 1 ; Zhou, Suiping 2 ; Luo, Jie 3 ; Li, Ling 1 ; Huang, Xin 4 ; Even, Zachary 1 ; Huang, He 1 ; Xu, Hai-Ming 4 ; Peng, Junmin 2 ; Lu, Lu 5 ; Wang, Xusheng 1 ;

作者机构： 1.Univ North Dakota, Dept Biol, Grand Forks, ND 58202 USA

2.St Jude Childrens Res Hosp, Ctr Prote & Metabol, Memphis, TN 38163 USA

3.Zhejiang Acad Agr Sci, Cent Lab, Hangzhou 310021, Peoples R China

4.Zhejiang Univ, Coll Agr & Biotechnol, Inst Bioinformat, Hangzhou 310058, Peoples R China

5.Univ Tennessee, Dept Genet Genom & Informat, Hlth Sci Ctr, Memphis, TN 38163 USA

关键词： Proteome; Mouse; Brain; Protein expression; Allele-specific expression; Protein regulation; Mass spectrometry

期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）

ISSN： 1471-2164

年卷期： 2021 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Natural variation in protein expression is common in all organisms and contributes to phenotypic differences among individuals. While variation in gene expression at the transcript level has been extensively investigated, the genetic mechanisms underlying variation in protein expression have lagged considerably behind. Here we investigate genetic architecture of protein expression by profiling a deep mouse brain proteome of two inbred strains, C57BL/6 J (B6) and DBA/2 J (D2), and their reciprocal F1 hybrids using two-dimensional liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) technology. Results By comparing protein expression levels in the four mouse strains, we observed 329 statistically significant differentially expressed proteins between the two parental strains and characterized the genetic basis of protein expression. We further applied a proteogenomic approach to detect variant peptides and define protein allele-specific expression (pASE), identifying 33 variant peptides with cis-effects and 17 variant peptides showing trans-effects. Comparison of regulation at transcript and protein levels show a significant divergence. Conclusions The results provide a comprehensive analysis of genetic architecture of protein expression and the contribution of cis- and trans-acting regulatory differences to protein expression.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台