文献类型： 外文期刊

作者： Ravelombola, Waltram ¹ ; Shi, Ainong ³ ; Bao-Lam Huynh ⁴ ; Qin, Jun ³ ; Xiong, Haizheng ³ ; Manley, Aurora ¹ ; Dong, Lingdi ⁶ ; Olaoye, Dotun ³ ; Bhattarai, Gehendra ³ ; Zia, Bazgha ³ ; Alshaya, Huda ³ ; Alatawi, Ibtisam ³ ;

作者机构： 1.Texas A&M AgriLife Res, 11708 Highway 70 South, Vernon, TX 76384 USA

2.Texas A&M Univ, Dept Soil & Crop Sci, 370 Olsen Blvd, College Stn, TX 77843 USA

3.Univ Arkansas, 316 Plant Sci Bldg PTSC, Fayetteville, AR 72701 USA

4.Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA

5.Hebei Acad Agr & Forestry Sci, North China Key Lab Biol & Genet Improvement Soyb, Minist Agr, Cereal & Oil Crop Inst,Natl Soybean Improvement C, Shijiazhuang 050031, Hebei, Peoples R China

6.Cereal & Hebei Acad Agr & Forestry Sci, 162 Hengshanjie St, Shijiazhuang 050035, Hebei, Peoples R China

期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）

ISSN： 1471-2164

年卷期： 2022 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Background Previous reports have shown that soil salinity is a growing threat to cowpea production, and thus the need for breeding salt-tolerant cowpea cultivars. A total of 234 Multi-Parent Advanced Generation Inter-Cross (MAGIC) lines along with their 8 founders were evaluated for salt tolerance under greenhouse conditions. The objectives of this study were to evaluate salt tolerance in a multi-parent advanced generation inter-cross (MAGIC) cowpea population, to identify single nucleotide polymorphism (SNP) markers associated with salt tolerance, and to assess the accuracy of genomic selection (GS) in predicting salt tolerance, and to explore possible epistatic interactions affecting salt tolerance in cowpea. Phenotyping was validated through the use of salt-tolerant and salt-susceptible controls that were previously reported. Genome-wide association study (GWAS) was conducted using a total of 32,047 filtered SNPs. The epistatic interaction analysis was conducted using the PLINK platform. Results Results indicated that: (1) large variation in traits evaluated for salt tolerance was identified among the MAGIC lines, (2) a total of 7, 2, 18, 18, 3, 2, 5, 1, and 23 were associated with number of dead plants, salt injury score, leaf SPAD chlorophyll under salt treatment, relative tolerance index for leaf SPAD chlorophyll, fresh leaf biomass under salt treatment, relative tolerance index for fresh leaf biomass, relative tolerance index for fresh stem biomass, relative tolerance index for the total above-ground fresh biomass, and relative tolerance index for plant height, respectively, with overlapping SNP markers between traits, (3) candidate genes encoding for proteins involved in ion transport such as Na+/Ca2+ K+ independent exchanger and H+/oligopeptide symporter were identified, and (4) epistatic interactions were identified. Conclusions These results will have direct applications in breeding programs aiming at improving salt tolerance in cowpea through marker-assisted selection. To the best of our knowledge, this study was one of the earliest reports using a MAGIC population to investigate the genetic architecture of salt tolerance in cowpea.