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Beware of Sealing Film of Petri Dishes!-Alters the Expression of a Large Number of Genes

文献类型： 外文期刊

作者： Ma, Yun 1 ; Li, Fang 1 ; Wang, Xuyang 1 ; Sun, Qingpeng 2 ; Wang, Ronghuan 1 ; Zhao, Jiuran 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Maize Res Inst, Beijing 100097, Peoples R China

2.Beijing Univ Agr, Coll Plant Sci & Technol, Beijing 100096, Peoples R China

关键词： sealingfilms; Arabidopsis seedlings; transcriptome; CO2 concentrations

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 12 期

页码：

收录情况： SCI

摘要： Arabidopsis seedlings grown in Petri dishes sealed with PE plastic wrap, PP parafilm, or NF surgical tape showed differences in growth, with PE plastic wrap resulting in a smaller size and fresh weight, followed by PP parafilm, compared to unsealed or NF surgical tape-sealed dishes. To investigate the basis of these phenotypic changes, transcriptome sequencing was performed. The results indicated that seedlings in dishes sealed with PE plastic wrap and PP parafilm exhibited over 1000 differentially expressed genes (DEGs) at 7 days. By 14 days, the number of DEGs had increased to over 2000 for each sealed condition. GO analysis showed that DEGs were commonly enriched in biological processes associated with the response to hypoxia under PE plastic wrap and PP parafilm sealing at both 7 and 14 days, as well as under NF surgical tape at 14 days. While O2 levels showed no significant differences between sealed and unsealed conditions, CO2 concentrations were notably lower in plates sealed with PE plastic wrap and PP parafilm. Furthermore, specific genes related to reduced size and delayed growth under sealed conditions were identified. In summary, sealing films negatively affect seedling growth, leading to significant shifts in gene expression profiles.

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