您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Integrated transcriptomic and proteomic analysis identified key folate biosynthesis gene in waxy maize

文献类型： 外文期刊

作者： Jiao, Yanyan 1 ; Shi, Yaxing 1 ; Dong, Hui 1 ; Pan, Yujing 1 ; Liu, Hui 1 ; Li, Zhiyong 1 ; Wang, Xiaqing 1 ; Li, Chunhui 1 ; Wang, Tianyi 1 ; Wang, Shuai 1 ; Wang, Ronghuan 1 ; Lu, Baishan 1 ; Song, Wei 1 ; Zhao, Jiuran 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Maize Res Inst, Beijing Key Lab Maize DNA Fingerprinting & Mol Bre, Beijing 100097, Peoples R China

关键词： Biofortification; Folate accumulation; Proteome; Transcriptome; Waxy maize

期刊名称：CROP JOURNAL （ 影响因子：5.6； 五年影响因子：6.0 ）

ISSN： 2095-5421

年卷期： 2025 年 13 卷 3 期

页码：

收录情况： SCI

摘要： One-third of the global population is affected by micronutrient deficiency, particularly folate. Although folate synthesis has been relatively well characterized, few folate-related genes in maize have been cloned, and the molecular mechanism regulating folate synthesis in maize remains unclear. In this study, transcriptome and proteome analyses of three waxy maize inbred lines with high, medium, and low folate contents were performed to identify key genes controlling folate biosynthesis. Pairwise comparisons revealed 21 differentially expressed genes and 20 differentially expressed proteins potentially associated with folate biosynthesis in the three lines. Six key folate-associated genes, ZmMocos2, ZmGGH, ZmADCL2, ZmCBR1, ZmSHMT, and ZmPurH, were identified. These genes encode enzymes that potentially function in folate biosynthesis. Functional validation of one of these genes, ZmADCL2, using an EMS mutant (Mut9264) showed that a 4-base insertion in an exon increased the folate content of fresh maize kernels 1.37-fold that of the wild type. ZmADCL2 was considered a potential target for generating maize lines with higher folate content. KEGG enrichment analysis of differentially expressed genes and proteins showed that several pathways in addition to folate biosynthesis were likely indirectly involved in folate metabolism and content (e.g., glycine, serine, and threonine metabolism; purine metabolism; cysteine and methionine metabolism; alanine, aspartate and glutamate metabolism; glutathione metabolism; and pyruvate metabolism. The transcriptome and proteomic data generated in this study will help to clarify the mechanisms underlying folate accumulation and aid breeding efforts to biofortify maize with folate. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台