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Carotene hydroxylase DcCYP97A3 affects carotenoid metabolic flow and taproot color by influencing the conversion of α-carotene to lutein in carrot

文献类型： 外文期刊

作者： Wang, Hui-Ru 1 ; Zhang, Rong-Rong 1 ; Wang, Ya-Hui 1 ; Zhou, Jian-Hua 2 ; Sun, Miao 1 ; Wang, Li-Xiang 1 ; Zhang, Yu-Qing 1 ; Liang, Yi 4 ; Li, Xiao-Jie 4 ; Xu, Zhi-Sheng 1 ; Ma, Jing 1 ; Liu, Hui 1 ; Tao, Jian-Ping 1 ; Xiong, Ai-Sheng 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort,Minist Agr & Rural Affairs, State Key Lab Crop Genet & Germplasm Enhancement &, Key Lab Biol & Germplasm Enhancement Hort Crops Ea, 1 Weigang, Nanjing 210095, Peoples R China

2.Inst Agr Sci & Technol Zhengzhou, 6 Changjiang Rd, Zhengzhou 450005, Peoples R China

3.Yancheng Teachers Univ, Coll Marine & Biol Engn, 2 Xiwang Rd, Yancheng 224002, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Vegetable Res Ctr, 11 Shuguang Garden Rd, Beijing 100097, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.5； 五年影响因子：9.1 ）

ISSN： 2662-6810

年卷期： 2025 年 12 卷 6 期

页码：

收录情况： SCI

摘要： The color diversity of non-purple carrot taproots is mainly affected by carotenoid species and content. Carrot cytochrome P450 carotene beta-ring hydroxylase (DcCYP97A3) may influence carotenoid accumulation in carrots; however, the roles of DcCYP97A3 in carrot remain unclear. Compared to the orange carrot 'Kurodagosun, KRD', the yellow carrot 'Yellowstone, YST' had greater relative transcript levels of DcCYP97A3. DcCYP97A3 was shown to catalyze the beta-ring hydroxylation of alpha-carotene to create zeaxanthin when it was expressed in Escherichia coli accumulating alpha- and beta-carotene. Expression of the DcCYP97A3 of 'YST' in DcCYP97A3 functionally deficient orange carrot 'KRD' resulted in yellow taproots, decreased alpha-carotene and beta-carotene content, decreased alpha-/beta-carotene ratio, and increased lutein content. In carrots overexpressing the DcCYP97A3 gene, the transcript levels of DcLCYE and DcLCYB1 were significantly upregulated and downregulated, respectively. Gene editing of DcCYP97A3 in 'YST' resulted in DcCYP97A3 knockout mutants with significantly reduced levels of lutein and beta-carotene and significantly upregulated transcript levels of DcCHXB2 and DcCCD4. These findings advance our knowledge of the molecular mechanisms behind carrot carotenoid metabolism.

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