The ammonium transporter AmtB is dispensable for the uptake of ammonium in the phototrophic diazotroph Rhodopseudomonas palustris
文献类型： 外文期刊
作者： Huang, Lu 1 ; Lu, Weilai 1 ; Yu, Yunkai 3 ; Qiu, Haoning 1 ; Zeng, Yan 1 ; Wang, Lida 3 ; Liu, Ying 1 ; Yan, Lei 4 ; Vincent, Yu 1 ; Zheng, Yanning 1 ;
作者机构： 1.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100101, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
3.Heilongjiang Acad Agr Sci, Qiqihar Branch, Qiqihar 161006, Peoples R China
4.Heilongjiang Bayi Agr Univ, Coll Life Sci & Biotechnol, Heilongjiang Prov Key Lab Environm Microbiol & Rec, Daqing 163319, Peoples R China
关键词： Ammonium uptake; AmtB; Raman spectroscopy; Photosynthetic diazotroph; Rhodopseudomonas palustris
期刊名称：ENVIRONMENTAL TECHNOLOGY & INNOVATION （ 影响因子：7.1； 五年影响因子：7.1 ）
ISSN： 2352-1864
年卷期： 2024 年 36 卷
页码：
收录情况： SCI
摘要： Ammonium (NH4+) 4 + ) transport across cell membranes plays an important role in assimilation or removal of the environmental nitrogen. The membrane protein AmtB has been considered to be the NH4+ 4 + transporter that is responsible for the NH4+ 4 + uptake. The phototrophic diazotroph Rhodopseudomonas palustris harboring two amtB genes has been widely used in wastewater treatment and bioremediation. However, the role of AmtB in NH4+ 4 + uptake remains unclear in R. palustris. . Here, we employed an innovative approach combining stable isotope probing (SIP) with Raman spectroscopy to determine the physiological functions of AmtB1 and AmtB2 in R. palustris. This powerful technique allowed us to investigate NH4+ 4 + uptake at the single-cell level. The generated R. palustris Delta amtB1 Delta amtB2 mutant lacking AmtB1 and AmtB2 proteins was still capable of utilizing 15 NH 4 + even the 15 NH 4 + concentration was as low as 5 mu M. These data demonstrate that both of the AmtB proteins are not essential for R. palustris to take up NH4+ 4 + regardless of environmental NH4+ 4 + levels. However, both AmtB1 and AmtB2 can contribute to NH4+ 4 + uptake under nitrogen- limiting conditions. Given that R. palustris primarily expresses AmtB2 in these conditions, AmtB2 plays a more important role in NH4+ 4 + uptake compared to AmtB1. In addition, transcriptomic analysis showed that the deletion of the amtB1 and amtB2 genes resulted in the upregulation of many transporter genes, providing potential targets for future investigation of alternative NH4+ 4 +
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