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Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis

文献类型： 外文期刊

作者： Li, Xiaoxia 1 ; Yang, Weiguang 2 ; Jia, Junting 3 ; Zhao, Pincang 4 ; Qi, Dongmei 1 ; Chen, Shuangyan 1 ; Cheng, Li 5 ; Cheng, Liqin 1 ; Liu, Gongshe 1 ;

作者机构： 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China

2.Helongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163000, Peoples R China

3.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Prov Key Lab Crop Germplasm Resources P, Guangzhou 510640, Peoples R China

4.Hebei Univ Econ & Business, Coll Management Sci & Engn, Shijiazhuang 050062, Hebei, Peoples R China

5.Inst Hulun Buir Forestry & Grassland Sci Res, Hunlunbeier 021008, Peoples R China

关键词： sheepgrass; chloroplast; salt stress; seed germination

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2021 年 12 卷 12 期

页码：

收录情况： SCI

摘要： Sheepgrass is a perennial native grass species in China, and it can tolerate high levels of salt stress with an aggressive and vigorous rhizome system. Many salt-stress-responsive genes have been identified in sheepgrass. In this study, we report the cloning and characterization of a novel salt-induced gene, LcSAIN3 (Leymus chinensis salt-induced 3), from sheepgrass. Expression analysis confirmed that LcSAIN3 was induced by PEG, ABA, and salt treatments, and the expression of LcSAIN3 was significantly increased in salt-tolerant germplasms under salt treatment. Subcellular localization analysis indicated that the GFP-LcSAIN3 protein was mainly localized in the chloroplasts. The heterologous expression of LcSAIN3 in Arabidopsis increased the seed germination rate of transgenic plants under salt, ABA, and mannitol treatments. The seedling survival rate, plant height, and fresh weight of the transgenic plants were higher than those of WT plants under salt stress. The overexpression of LcSAIN3 caused a relatively high accumulation of free proline, enhanced SOD activity, and led to the upregulation of several stress-responsive genes such as AtRD26, AtRD29B, AtSOS1, and AtP5CS1. These results suggest that LcSAIN3 could be a potential target for molecular breeding to improve plants' salt tolerance.

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