文献类型： 外文期刊

作者： Chen, Chen ¹ ; Lu, Li-Li ¹ ; Ma, Shu-Ya ² ; Zhao, Yan-Peng ¹ ; Wu, Na ¹ ; Li, Wen-Jie ¹ ; Ma, Li ⁴ ; Kong, Xian-Hui ⁴ ; Xie, Zong-Ming ⁵ ; Hou, Yu-Xia ¹ ;

作者机构： 1.Zhengzhou Univ, Sch Agr Sci, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou 450001, Henan, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Peoples R China

3.China Agr Univ, Coll Sci, Beijing 100193, Peoples R China

4.Xinjiang Prod & Construct Corps, Agr Sci Inst, Div 3, Tumushuke 843901, Xinjiang, Peoples R China

5.Xinjiang Acad Agr & Reclamat Sci, Xinjiang Prod & Construct Grp, Key Lab Crop Germplasm Enhancement & Gene Resource, Shehezi 832000, Xinjiang, Peoples R China

6.Chinese Acad Agr Sci, Natl NanfanResearch Inst Sanya, Sanya 572024, Hainan, Peoples R China

关键词： GRAS; PAT1; GhSCL13-2A; Verticillium dahliae; Upland cotton

期刊名称：PLANT CELL REPORTS （ 2022影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0721-7714

年卷期： 2023 年 42 卷 3 期

页码：

收录情况： SCI

摘要： Key messageGhSCL13-2A, a member of the PAT1 subfamily in the GRAS family, positively regulates cotton resistance to Verticillium dahliae by mediating the jasmonic acid and salicylic acid signaling pathways and accumulation of reactive oxygen species.Verticillium wilt (VW) is a devastating disease of upland cotton (Gossypium hirsutum) that is primarily caused by the soil-borne fungus Verticillium dahliae. Scarecrow-like (SCL) proteins are known to be involved in plant abiotic and biotic stress responses, but their roles in cotton defense responses are still unclear. In this study, a total of 25 GhPAT1 subfamily members in the GRAS family were identified in upland cotton. Gene organization and protein domain analysis showed that GhPAT1 members were highly conserved. GhPAT1 genes were widely expressed in various tissues and at multiple developmental stages, and they were responsive to jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) signals. Furthermore, GhSCL13-2A was induced by V. dahliae infection. V. dahliae resistance was enhanced in Arabidopsis thaliana by ectopic overexpression of GhSCL13-2A, whereas cotton GhSCL13-2A knockdowns showed increased susceptibility. Levels of reactive oxygen species (ROS) and JA were also increased and SA content was decreased in GhSCL13-2A knockdowns. At the gene expression level, PR genes and SA signaling marker genes were down-regulated and JA signaling marker genes were upregulated in GhSCL13-2A knockdowns. GhSCL13-2A was shown to be localized to the cell membrane and the nucleus. Yeast two-hybrid and luciferase complementation assays indicated that GhSCL13-2A interacted with GhERF5. In Arabidopsis, V. dahliae resistance was enhanced by GhERF5 overexpression; in cotton, resistance was reduced in GhERF5 knockdowns. This study revealed a positive role of GhSCL13-2A in V. dahliae resistance, establishing it as a strong candidate gene for future breeding of V. dahliae-resistant cotton cultivars.