Developing a mutant strain of Pseudomonas composti ODT-54 for enhanced production of Psl extracellular polysaccharide
文献类型： 外文期刊
作者： Wu, Jieer 1 ; Han, Feng 3 ; Yu, Yongxin 1 ; Wang, Yongjie 1 ;
作者机构： 1.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Ocean & Polar Fisheries, Shanghai, Peoples R China
4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai, Peoples R China
关键词：
期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.6； 五年影响因子：2.8 ）
ISSN： 0302-8933
年卷期： 2025 年 207 卷 4 期
页码：
收录情况： SCI
摘要： In our previous study, a strain of Pseudomonas composti ODT-54 was isolated from the oyster digestive tissues. This strain is capable of producing Psl exopolysaccharides (EPS), which are directly involved in the specific binding to norovirus GII.6 P proteins. Here, we constructed an ODT-54 Psl EPS overexpression strain ODT-54 psl(+) by substituting an exogenous arabinose-inducible promoter for the native promoter of the psl gene cluster. ODT-54 psl(+) produced 50-70% more Psl EPS in FAB medium with 20 g/L arabinose induction compared to ODT-54, according to ELISA analyses. Furthermore, a 50% increase in binding to GII.6 P proteins was observed for ODT-54 psl(+). These findings confirmed the success of the construction of the ODT-54 Psl EPS overexpression strain. This work provides important experimental material and helps further explore the structure of Psl EPS from P. composti.
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