Effects of neuropeptide F signaling on feeding, growth and development of Plutella xylostella (L.) larvae
文献类型： 外文期刊
作者： Shen, Cheng 1 ; Wu, Jiayi 1 ; Huang, Ziyan 1 ; He, Meng 1 ; Chen, Wei 1 ; Ilyas, Naila 1 ; Zhang, Xiao 1 ; Chen, Chengyu 1 ; Xu, Chongxin 1 ; Xie, Yajing 1 ; Wang, Yun 4 ; Liu, Yuan 1 ; Liu, Xianjin 1 ; Zhu, Qing 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual & Safety, Key Lab Control Technol & Stand Agroprod Safety &, Nanjing, Peoples R China
2.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China
3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Peoples R China
4.Jinling Inst Technol, Coll Hort, Nanjing, Peoples R China
关键词： Plutella xylostella; Neuropeptide F signaling; Feeding regulation
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 293 卷
页码：
收录情况： SCI
摘要： The neuropeptide F (NPF) signaling, comprising NPF and neuropeptide F receptor (NPFR), role in regulating insect behaviors and physiological processes. We cloned the genes encoding NPF and NPFR from Plutella xylostella, a notorious pest of cruciferous crops. Notably, the NPF gene produced two splicing variants, Px-NPF1 and Px-NPF2, with distinct expression patterns. Conserved C-terminal RPRFamide motif and seven transmembrane alpha-helics were observed in Px-NPF and in Px-NPFR, respectively. Px-NPF and Px-NPFR are widely expressed across all larval instars and are predominantly localized in the brain and midgut. The transcriptional levels of Px-NPF1 and Px-NPFR increased and reached maximum at 8 h after food deprived. RNA interference targeting Px-NPF and Px-NPFR resulted in smaller body size, delayed larval growth and alterations in energy metabolism. Specific binding assays revealed stronger binding affinity of Px-NPF1 with Px-NPFR than Px-NPF2. Three-dimensional models of the Px-NPF-NPFR complexes via AlphaFold 3 highlighted the critical roles of two highly conserved residues across insects, Tyr46 and His196, in interaction. Our findings underscore the potential of targeting NPF-NPFR signaling for P. xylostella controlling and contributing to developing novel pest management strategies.
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