Development of real-time recombinase polymerase amplification (RPA) and RPA combined with lateral flow dipstick (LFD) assays for the rapid and sensitive detection of cyprinid herpesvirus 3
文献类型： 外文期刊
作者： Li, Yingying 1 ; Li, Ruifan 1 ; Mo, Xubing 1 ; Wang, Yingying 1 ; Yin, Jiyuan 1 ; Bergmann, Sven M. 2 ; Ren, Yan 1 ; Pan, Houjun 1 ; Shi, Cunbin 1 ; Zhang, Defeng 1 ; Wang, Qing 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou, Peoples R China
2.Fed Res Inst Anim Hlth, Inst Infectol, Friedrich Loffler Inst FLI, Germany Reference Lab KHVD, Greifswald Insel Riems, Germany
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China
关键词： cyprinid herpesvirus 3(CyHV-3); real-time RPA; RPA-LFD; sera
期刊名称：JOURNAL OF FISH DISEASES （ 影响因子：2.5； 五年影响因子：2.6 ）
ISSN： 0140-7775
年卷期： 2024 年
页码：
收录情况： SCI
摘要： In this issue, we established rapid, cost-effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA-LFD) and real-time RPA for cyprinid herpesvirus 3(CyHV-3), and evaluated their sensitivity, specificity, and applicability, the real-time RPA method could achieve sensitive diagnosis of CyHV-3 within 1.3 copies per reaction, respectively. The real-time RPA method is 10-fold more sensitive than RPA-LFD method. The exact number of CyHV-3 can be calculated in each sample by real-time RPA. The sera from koi also can be tested in these methods. In addition, no cross-reaction was observed with other related pathogens, including carp oedema virus (CEV), spring viraemia of carp virus (SVCV), cyprinid herpesvirus 1(CyHV-1), cyprinid herpesvirus 2(CyHV-2), type I grass carp reovirus (GCRV-I), type II GCRV (GCRV-II), type III GCRV (GCRV-III), and Aeromonas hydrophila.
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