Long non-coding RNA NEAT1 promotes mouse granulosa cell proliferation and estradiol synthesis by sponging miR-874-3p
文献类型： 外文期刊
作者： Zhang, Pengju 1 ; Gao, Jinliang 2 ; Lin, Shan 3 ; Lin, Guangyu 4 ; Wang, Weixia 1 ; Tan, Chengcheng 1 ; Liu, Xiaohui 1 ; Li, Xintao 1 ; Zhang, Lichun 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Anim Biotechnol, Changchun 130000, Jilin, Peoples R China
2.Changchun Univ Chinese Med, Affiliated Hosp, Dept Rheumatol, Changchun 130117, Jilin, Peoples R China
3.Jilin Univ, China Japan Union Hosp, Med Dept, Changchun 130033, Jilin, Peoples R China
4.Anim Husb Informat Ctr Jilin Prov, Changchun 130000, Jilin, Peoples R China
5.Jilin Acad Agr Sci, Inst Anim Biotechnol, 1363 Shengtai St,Jingyue, Changchun 130000, Jilin, Peoples R China
关键词： long non-coding RNA; nuclear-enriched abundant transcript 1; mouse granulosa cell; apoptosis; estradiol synthesis
期刊名称：EXPERIMENTAL AND THERAPEUTIC MEDICINE （ 影响因子：2.751； 五年影响因子：2.475 ）
ISSN： 1792-0981
年卷期： 2023 年 25 卷 1 期
页码：
收录情况： SCI
摘要： It has been reported that long non-coding RNA nuclear-enriched abundant transcript 1 (NEAT1) is involved in follicular growth and multiple ovarian diseases, but not the physiological function of NEAT1 in mouse granulosa cells (mGCs). Therefore, the aim of the present study was to investigate the biological roles and regulatory mechanisms of NEAT1 in mGCs. The biological effects of NEAT1 on mGCs proliferation, apoptosis, production of 17 beta-Estradiol (E2) and progesterone (P4) were investigated using MTS, flow cytometry and enzyme-linked immunosorbent assays, respectively. The association between NEAT1 and microRNA (miR)-874-3p was verified using luciferase reporter assay and RNA immunoprecipitation analysis. The results demonstrated that the knockdown of NEAT1 in mGC cells significantly promoted mGCs cell proliferation, inhibited apoptosis and increased the production of E2 and P4 in mGCs. The interference-mediated effect of NEAT1 on mGCs could be partially reversed by the downregulation of miR-874-3p. Overall, these results indicated that NEAT1 served as a competing endogenous RNA by competitively binding with miR-874-3p, thereby modulating mGCs proliferation and the production of E2 and P4 in mGCs.
- 相关文献
作者其他论文 更多>>
-
miR-370-3p Inhibited the Proliferation of Sheep Dermal Papilla Cells by Inhibiting the Expression of SMAD4
作者：Fu, Jiaqi;Wang, Dan;Liu, Wenqing;Qi, Yu;Zhang, Caihong;Li, Huansong;Cai, Jinshun;Ji, Shuang;Sun, Fuliang;Wang, Dan;Qi, Yu;Zhang, Caihong;Zhang, Lichun
关键词：
miR-370-3p ;SMAD4 ; dermal papilla cells; cell phenotype -
Molecular patterns of matrix protein 1 (M1): A strong predictor of adaptive evolution in H9N2 avian influenza viruses
作者：Zhu, Yanting;Cong, Yulin;Sheng, Shouzhi;Liu, Chang;Jiang, Jianfeng;Li, Siqi;Gao, Chao;Zhao, Jinghui;Cong, Yanlong;Zhu, Yanting;Cong, Yulin;Sheng, Shouzhi;Liu, Chang;Jiang, Jianfeng;Li, Siqi;Gao, Chao;Zhao, Jinghui;Cong, Yanlong;Sun, Yixue;Zhang, Pengju
关键词：matrix protein M1; genetic pattern; virus replication; epidemiological dominance
-
Alpha-lipoic acid improves cryopreservation of rooster semen by reducing oxidative stress
作者：Chen, Xiaoxin;Liu, Jianqiang;Liu, Yi;Li, Xu;An, Dingjie;Liu, Xiaohui;Zhang, Lichun
关键词：alpha-lipoic acid; antioxidant capacity; semen freezing; rooster sperm ultramicrostructure
-
Hemagglutinin glycosylation pattern-specific effects: implications for the fitness of H9.4.2.5-branched H9N2 avian influenza viruses
作者：Sun, Yixue;Sun, Yixue;Zhu, Yanting;Sheng, Shouzhi;Guan, Zhenhong;Cong, Yanlong;Sun, Yixue;Zhu, Yanting;Sheng, Shouzhi;Guan, Zhenhong;Cong, Yanlong;Zhang, Pengju
关键词：Avian influenza; h9.4.2.5 branch; hemagglutinin; glycosylation; adaptive phenotype
-
The comparison of meat yield, quality, and flavor between small-tailed Han sheep and two crossbred sheep and the verification of related candidate genes
作者：Xiao, Cheng;Liu, Yu;Zhao, Wenjun;Liang, Yingjia;Cui, Chao;Yang, Shaoying;Fang, WenWen;Miao, Lisheng;Yuan, Zhiyu;Zhai, Bo;Zhao, Zhongli;Zhang, Lichun;Ma, Huihai;Jin, Haiguo;Cao, Yang;Xiao, Cheng;Xiao, Cheng;Zhao, Wenjun;Lin, Zihan
关键词：small-tailed Han sheep; crossbred sheep; meat yield; quality; volatile compound; PDK4 gene; TMEM273 gene
-
The IL-33/ST2 Axis Affects Adipogenesis Through Regulating the TRAF6/RelA Pathway
作者：Cao, Shujun;Qin, Xuyong;Li, Chengping;Ren, Shizhong;Zhou, Wenhao;Zhao, Meiman;Zhou, Guoli;Zhang, Lichun
关键词：IL-33/ST2 axis; signaling pathways; adipogenesis; TRAF6; RelA
-
Analysis of the Long Non-Coding and Messenger RNA Expression Profiles in the Skin Tissue of Super Merino and Small-Tailed Han Sheep
作者：Fu, Jiaqi;Zhang, Xinyu;Wang, Dan;Liu, Wenqing;Zhang, Caihong;Wang, Wei;Fan, Wei;Sun, Fuliang;Zhang, Lichun
关键词：super merino sheep; small-tailed han sheep; lncRNA; mRNA; RNA sequencing; immunohistochemistry
