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Genetic architecture of embryogenic callus induction in maize from the perspective of population genomics

文献类型： 外文期刊

作者： Dai, Liqiang 1 ; Han, Siping 1 ; Zhang, Yan 1 ; Hao, Dongyun 1 ;

作者机构： 1.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Peoples R China

关键词： Embryogenic callus; Maize inbred lines; Genome-wide association study (GWAS); Allelic variation; Candidate genes

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.726； 五年影响因子：2.859 ）

ISSN： 0167-6857

年卷期： 2022 年 150 卷 2 期

页码：

收录情况： SCI

摘要： Embryogenic callus induction (ECI) is an initial step in plant cell totipotency that is essential for somatic cell regeneration. Identifying genes associated with embryogenic callus (EC) formation is critical to understand the molecular mechanism of totipotency and crop biotechnology breeding. In this study, we used a population of 177 maize (Zea mays L.) inbred lines as an association panel to investigate the percentage of embryogenic callus induction (%ECI) from their immature embryos in two independent seasons. Whole-genome resequencing of this population produced 3,786,431 high-quality single nucleotide polymorphisms (SNPs), after which a genome-wide association study (GWAS) was performed for the ECI trait. The results showed that %ECIs varied greatly from 0.00 to 98.15% among the tested inbred lines, with a broad-sense heritability of 0.71. Using the FarmCPU method, we detected 104 candidate genes within the linkage disequilibrium (LD) blocks of 57 significant SNPs (P < 0.000001). Among these genes, ZmCLE4B and Ereb83 harbored significant sense mutations, and inbred lines with different alleles of these genes showed significant differences in ECI (P < 0.00001). Expression analyses of these two genes in five EC-induction stages in two ECI-contradictory inbred lines revealed that different alleles of the genes behave divergently in response to callus induction culture. Additionally, using an intermate population, we noted that ZmCLE4B plants with different homozygous alleles were phenotypically different (P < 0.01). Taken together, the current results suggest that ZmCLE4B is likely a major factor in ECI. Key message A total of 104 candidate genes for embryogenic callus induction (ECI) were identified, and molecular evidence showed that the ZmCLE4B gene is likely a major factor in ECI.

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