文献类型： 外文期刊

作者： Ye, Ting ¹ ; Su, Hailan ² ; Zheng, Guohua ³ ; Meng, Hongyan ¹ ; Wang, Wenhua ¹ ; Guo, Ying ¹ ;

作者机构： 1.Fujian Inst Subtrop Bot, Fujian Key Lab Subtrop Plant Physiol & Biochem, Xiamen 361006, Peoples R China

2.Fujian Acad Agr Sci, Inst Crop Sci, Fujian Germplasm Resources Ctr, Fuzhou 350000, Peoples R China

3.Xiamen Overseas Chinese Subtrop Plant Intro Garden, Plant Intro & Quarantine Base & Plant Prod Key Lab, Xiamen 361002, Peoples R China

关键词： Paris polyphylla; microbiome; transcriptome; metabolome; root rot disease

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Root rot of Paris polyphylla has received widespread attention due to its threat to yield and leads to serious economic losses. However, the relationship among the rhizosphere microbial community, metabolites and root rot disease remained largely unexplored. Herein, we used integrated 16S rRNA, ITS, RNA sequencing and UPLC-MS/MS to systematically investigate the differences between healthy and diseased P. polyphylla. We found that root rot reduced the microbial diversity in the diseased P. polyphylla compared with the healthy control. The relative abundance of the bacterial phylum Actinobacteria increased in the diseased rhizome of P. polyphylla. For the fungal community, root rot disease contributed to an increased relative abundance of Ascomycota and decreased Glomeromycota at the phylum level. The transcriptomic results showed that the differently expressed genes were significantly enriched in the "Biosynthesis of various alkaloids", "flavonoid biosynthesis" and "isoflavonoid biosynthesis" and "Phenylpropanoid biosynthesis" was dramatically enriched in healthy P. polyphylla compared with that in diseased P. polyphylla. Likewise, the metabolomic results showed that the biosynthesis of secondary metabolites and metabolic pathways was found to be significantly enriched by differential metabolites. Taken together, the study of combining metabolomics with microbiomes can help us enhance our understanding of the mechanisms of plant resistance to root rot disease, thereby discovering specific metabolites and microorganisms that can resist pathogen infection in P. polyphylla.