CRISPR/Cas knockout of the NADPH oxidase gene OsRbohB reduces ROS overaccumulation and enhances heat stress tolerance in rice
文献类型： 外文期刊
作者： Liu, Xiaolong 1 ; Ji, Ping 1 ; Liao, Jingpeng 1 ; Duan, Ximiao 1 ; Luo, Zhiyang 1 ; Yu, Xin 2 ; Jiang, Chang-Jie 3 ; Xu, Chen 4 ; Yang, Hongtao 1 ; Peng, Bo 1 ; Jiang, Kai 1 ;
作者机构： 1.Yichun Univ, Coll Life Sci & Resources & Environm, Yichun, Jiangxi, Peoples R China
2.Yichun Univ, Coll Chem & Bioengn, Yichun, Jiangxi, Peoples R China
3.Shandong Acad Agr Sci, Shandong Rice Res Inst, Jinan, Shandong, Peoples R China
4.Jilin Acad Agr Sci, Inst Agr Resources & Environm, Changchun, Jilin, Peoples R China
关键词：
Rice (
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）
ISSN： 1467-7644
年卷期： 2025 年 23 卷 2 期
页码：
收录情况： SCI
摘要： Heat stress (HS) has become a major factor limiting crop yields worldwide. HS inhibits plant growth by ROS accumulation, and NADPH oxidases (Rbohs) are major ROS producers in plants. Here, we show that CRISPR/Cas knockout of the OsRbohB (OsRbohB-KO) significantly increased rice tolerance to HS imposed at various different growth stages. We produced OsRbohB-KO and OsRbohB-overexpression (OsRbohB-OE) lines in a japonica cultivar, Nipponbare. Compared with nontransgenic wild-type (WT) plants, the OsRbohB-KO lines showed a significant increase in chlorophyll contents (5.2%-58.0%), plant growth (48.2%-65.6%) and grain yield (8.9%-20.5%), while reducing HS-induced ROS accumulation in seeds (21.3%-33.0%), seedlings (13.0%-30.4%), anthers (13.1%-20.3%) and grains (9.7%-22.1%), under HS conditions. Analysis of yield components revealed that the increased yield of OsRbohB-KO plants was due to increased starch synthetase activity, spikelets per panicle (2.0%-9.3%), filled spikelets (4.8%-15.5%), percentage of filled spikelets (2.4%-6.8%) and 1000-grain weight (2.9%-7.4%) under HS conditions during the reproductive stage. Grain milling and appearance quality, and starch content were also significantly increased in OsRbohB-KO plants under HS conditions during the mature stage. Furthermore, OsRbohB-KO significantly upregulated the expression levels of heat shock-related genes, OsHSP23.7, OsHSP17.7, OsHSF7 and OsHsfA2a, in rice seedlings and grains under long-term HS conditions. Conversely, OsRbohB-OE resulted in phenotypes that were opposite to OsRbohB-KO in most cases. Our results suggest that suppression of OsRbohB provides an effective approach for alleviating heat damage and improving grain yield and quality of rice under long-term HS conditions.
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