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Dynamic Pollen-Stigma Coordination in Dendrobium Hybridization: A Strategy to Maximize Fruit Set and Hybrid Seed Viability

文献类型： 外文期刊

作者： Wu, Qian 1 ; Qian, Yanbing 1 ; Guan, Ao 1 ; Yue, Yan 1 ; Li, Zongyan 1 ; Dunn, Bruce 2 ; Yang, Jianwei 3 ; Yi, Shuangshuang 4 ; Liao, Yi 4 ; Yin, Junmei 4 ;

作者机构： 1.Southwest Forestry Univ, Landscape Architecture & Hort Fac, Kunming 650224, Peoples R China

2.Oklahoma State Univ, Dept Hort & Landscape Architecture, Stillwater, OK 74075 USA

3.West Yunnan Univ Appl Sci, Coll Tea Puer, Puer 665099, Peoples R China

4.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China

5.Chinese Acad Trop Agr Sci, Sanya Res Inst, Engn Technol Res Ctr Trop Ornamental Plant Germpla, Sanya 571101, Peoples R China

关键词： Dendrobium; interspecific hybridization; developmental traits; pollen; sigma

期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN：

年卷期： 2025 年 11 卷 5 期

页码：

收录情况： SCI

摘要： This study investigated dynamic pollen-stigma coordination to optimize interspecific hybridization in Dendrobium using D. 'Burana Jade' as the maternal parent and eight wild species as pollen donors. Stigma receptivity was comprehensively evaluated using a multi-indicator approach, including morphological characterization (crystal secretion and bulging papillae), histochemical benzidine-H2O2 staining, and enzymatic activity profiling (esterase and superoxide dismutase). Concurrently, pollen viability was assessed through TTC testing coupled with ultrastructural observations. Results identified a critical synchronization window: pollen viability peaked at 1-3 days post anthesis (DPA) or during the mid-anthesis phase, while stigmas exhibited maximal receptivity when secretory activity and antioxidant enzyme levels significantly increased. Using stage-specific pollination criteria, 8.4% of crosses (8/95) produced viable fruits, outperforming empirical methods by 2.8-fold. D. 'Burana Jade' showed cross-compatibility with four Dendrobium species (D. aphyllum, D. chrysotoxum, D. hercoglossum, D. thyrsiflorum), with D. thyrsiflorum hybrids achieving 54.81% embryogenesis and 22.38% germination. Three compatible combinations germinated successfully in vitro within 45-55 days on 1/4 MS medium supplemented with 20 g/L sucrose, 1 g/L tryptone, 180 mL/L coconut water, and 2.2 g/L Phytagel. Our findings establish that synchronizing pollen viability windows with stigma receptivity phases significantly enhances fruit set and hybrid seed viability, providing a phenology-driven strategy to overcome reproductive barriers in orchid breeding programs. This study provides key physiological criteria for Dendrobium hybridization, though their applicability to other orchids needs validation. Future multi-omics studies should explore cross-species compatibility mechanisms.

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