Isolation and Functional Analysis of the DhMYB2 and DhbHLH1 Promoters from Phalaenopsis-Type Dendrobium Involved in Stress Responses and Tissue-Specific Expression
文献类型： 外文期刊
作者： Wang, Yachen 1 ; Li, Chonghui 2 ; Zhu, Wenjuan 3 ; Li, Yamei 2 ; Song, Xiqiang 4 ; Yin, Junmei 2 ;
作者机构： 1.Hainan Open Univ, Sch Agr & Rural Affairs, Haikou 570208, Peoples R China
2.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Lab Crop Gene Resources & Germplasm Enhancement So, Minist Agr,Engn Technol Res Ctr Trop Ornamental Pl, Haikou 571101, Peoples R China
3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou 310024, Peoples R China
4.Hainan Univ, Sch Trop Agr & Forestry, Germplasm Innovat Trop Special Forest Trees & Orna, Minist Educ, Haikou 570100, Peoples R China
5.Chinese Acad Trop Agr Sci, Sanya Res Inst, Natl Key Lab Trop Crop Breeding, Sanya 572024, Peoples R China
关键词：
期刊名称：HORTICULTURAE （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 11 卷 5 期
页码：
收录情况： SCI
摘要： Phalaenopsis-type Dendrobium (Den-Phals) is a commercially valuable orchid, with floral color being key to its market appeal. Despite the significance of anthocyanin biosynthesis in color development, its transcriptional regulation in Den-Phals remains unclear. This study functionally characterized the promoters of DhMYB2 and DhbHLH1, two key transcription factors involved in anthocyanin biosynthesis. A 1864 bp DhMYB2 promoter and a 1995 bp DhbHLH1 promoter were isolated using genome walking. Bioinformatics analysis identified cis-acting elements associated with abiotic stress responses, phytohormone signaling, and floral-specific regulation. 5 '-Deletion analysis in tobacco leaves identified core regulatory regions for the DhMYB2 promoter (-1864 to -937 bp) and DhbHLH1 promoter (-1995 to -924 bp). GUS staining and activity assays demonstrated that the activities of the DhMYB2 and DhbHLH1 promoters were significantly increased under treatments of long light, low temperature, drought, salicylic acid (SA), and abscisic acid (ABA), while the DhbHLH1 promoter was also induced by methyl jasmonate (MeJA) and indole-3-acetic acid (IAA). Furthermore, promoter activity of DhMYB2 and DhbHLH1 was detected only in transgenic Arabidopsis flowers, suggesting that these promoters exhibit floral-specific activity. This study provides the first functional analysis of Den-Phals anthocyanin promoters, revealing their stress-responsive nature and floral specificity, which will facilitate molecular breeding of novel orchid cultivars.
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