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Quantitative Analysis of Anthocyanins in Grapes by UPLC-Q-TOF MS Combined with QAMS

文献类型： 外文期刊

作者： Li, Xue 1 ; Wang, Wei 1 ; Sun, Suling 1 ; Wang, Junhong 1 ; Zhu, Jiahong 1 ; Liang, Feng 4 ; Zhang, Yu 1 ; Hu, Guixian 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Hangzhou 310021, Peoples R China

2.Minist Agr & Rural Affairs China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Peoples R China

3.Zhejiang Prov Key Lab Food Safety, Hangzhou 310021, Peoples R China

4.Bohai Univ, Coll Food Sci & Technol, Jinzhou 121013, Peoples R China

关键词： anthocyanins; grapes; UPLC-Q-TOFMS; QAMS

期刊名称：SEPARATIONS （ 影响因子：3.344； 五年影响因子：2.943 ）

ISSN：

年卷期： 2022 年 9 卷 6 期

页码：

收录情况： SCI

摘要： A method for quantifying the anthocyanins in grapes was firstly developed by ultrahigh performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOFMS) combined with quantitative analysis of multi-components by single marker (QAMS). A total of 10 main anthocyanins were analyzed by using peonidin 3-O-glucoside as the reference standard. The accuracy of this method was evaluated by an established and validated external standard quantification method with 10 reference compounds. The standard method difference (SMDs) of the quantification results between QAMS and the external standard methodwasless than 15%. Furthermore, the QAMS method was used to analyzefour batches of grapes and the data was compared with those obtained using the external standard method. No significant difference wasobtained in the results obtained by both methods. These results indicated that the QAMS method could accurately determine the anthocyanins in grapes. This method can provide a basis to address the absence of reference standards for analyzing anthocyanins in other foods.

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