A phosphorylation-regulated NPF transporter determines salt tolerance by mediating chloride uptake in soybean plants
文献类型： 外文期刊
作者： Wu, Yunzhen 1 ; Yuan, Jingya 1 ; Shen, Like 1 ; Li, Qinxue 3 ; Li, Zhuomeng 1 ; Cao, Hongwei 1 ; Zhu, Lin 1 ; Liu, Dan 1 ; Sun, Yalu 1 ; Jia, Qianru 4 ; Chen, Huatao 4 ; Wang, Wubin 2 ; Kudla, Joerg 5 ; Zhang, Wenhua 1 ; Gai, Junyi 2 ; Zhang, Qun 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China
2.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, State Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Peoples R China
3.Int Campus Zhejiang Univ, Prov Int Sci & Technol Cooperat Base Engn Biol, Hangzhou 314400, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China
5.Univ Munster, Inst Biol & Biotechnol Pflanzen IBBP, Munster, Germany
关键词： Chloride Homeostasis; NPF Transporters; Phosphorylation; Soybean; Salt Tolerance
期刊名称：EMBO JOURNAL （ 影响因子：8.3； 五年影响因子：10.6 ）
ISSN： 0261-4189
年卷期： 2025 年 44 卷 3 期
页码：
收录情况： SCI
摘要： Chloride (Cl-) ions cause major damage to crops in saline soils. Understanding the key factors that influence Cl- uptake and translocation will aid the breeding of more salt-tolerant crops. Here, using genome-wide association study and transcriptomic analysis, we identified a NITRATE TRANSPORTER 1 (NRT1)/PEPTIDE TRANSPORTER family (NPF) protein, GmNPF7.5, as the dominant gene locus influencing Cl- homeostasis in soybean (Glycine max). A natural SNP variation resulted in two haplotypes (GmNPF7.5HapA and GmNPF7.5HapB), which was associated with Cl- content. GmNPF7.5HapA mediated Cl- or nitrate (NO3-) uptake in a pH-dependent manner and exhibited higher permeability for Cl- over NO3-. The suppression of GmNPF7.5HapA expression decreased Cl- accumulation and salt damage in plants, whereas its overexpression showed the opposite effects. The elite haplotype GmNPF7.5HapB diminished Cl- transport activity independently from NO3- permeability, thus enhancing soybean salt tolerance. Furthermore, the protein kinase GmPI4K gamma 4 could phosphorylate GmNPF7.5, which repressed Cl- uptake without affecting NO3- permeability. Our findings define a regulatory mechanism for Cl- control under NaCl stress, providing a strategy for the improvement of salt tolerance in soybean plants.
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