Cloning and characterization of one novel PR1 gene from Coptis chinensis responding to southern blight
文献类型： 外文期刊
作者： Xu, Ran 1 ; Li, Xiaohan 1 ; Ming, Yue 1 ; Wang, Bo 2 ; Bi, Kai 1 ; Wang, Limei 1 ; Wang, Hongxun 1 ; Zhu, Wenjun 1 ; You, Jingmao 3 ;
作者机构： 1.Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Peoples R China
2.Hubei Inst Durg Control, Wuhan 430023, Peoples R China
3.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi 445000, Peoples R China
关键词： Coptis chinensis; Plant defense; PR1 protein; Southern blight; Transcriptome
期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：3.3； 五年影响因子：3.2 ）
ISSN： 0885-5765
年卷期： 2025 年 138 卷
页码：
收录情况： SCI
摘要： Southern blight, caused by Sclerotium rolfsii, is a major threat to Coptis chinensis production, but the plant's defense mechanisms and resistance genes are poorly understood. In this study, transcriptomic analysis of healthy and infected C. chinensis identified the key resistance gene Coptis chinensis pathogenesis-related protein 1 (CcPR1). The full sequence of CcPR1 was cloned and analyzed bioinformatically. qRT-PCR showed that CcPR1 expression was significantly upregulated in pathogen-infected plants, highlighting its role in disease resistance. Additionally, anti-fungal assays of the recombinant CcPR1 protein from Escherichia coli confirmed its activity. Subcellular localization placed the protein in the extracellular matrix. CcPR1 overexpression in Nicotiana benthamiana leaves significantly inhibited S. rolfsii and Botrytis cinerea growth, suggesting broad-spectrum resistance to fungal pathogens. This study advances the understanding of CcPR1 and its role in C. chinensis defense against southern blight.
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