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Stress response and tolerance mechanisms of NaHCO3 exposure based on biochemical assays and multi-omics approach in the liver of crucian carp (Carassius auratus)

文献类型： 外文期刊

作者： Wei, Xiao-feng 1 ; Liu, Ying-jie 1 ; Li, Shan-wei 1 ; Ding, Lu 1 ; Han, Shi-cheng 1 ; Chen, Zhong-xiang 1 ; Lu, Hang 2 ; Wang, Peng 1 ; Sun, Yan-chun 1 ;

作者机构： 1.Minist Agr & Rural Areas, Heilongjiang River Fisheries Res Inst, Lab Qual & Safety Risk Assessment Aquat Prod Harbi, Chinese Acad Fishery Sci, Harbin 150070, Peoples R China

2.Dalian Ocean Univ, Coll Food Sci & Engn, Dalian 116023, Peoples R China

3.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

关键词： Saline-alkaline stress; Fish; Liver; Biochemical analysis; Metabolomics; Transcriptomics

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 253 卷

页码：

收录情况： SCI

摘要： The development and utilization of saline-alkaline water, an important backup resource, has received wide-spread attention. However, the underuse of saline-alkaline water, threatened by the single species of saline-alkaline aquaculture, seriously affects the development of the fishery economy. In this work, a 30-day NaHCO3 stress experimental study combined with analyses of untargeted metabolomics, transcriptome, and biochemical approaches was conducted on crucian carp to provide a better understanding of the saline-alkaline stress response mechanism in freshwater fish. This work revealed the relationships among the biochemical pa-rameters, endogenous differentially expressed metabolites (DEMs), and differentially expressed genes (DEGs) in the crucian carp livers. The biochemical analysis showed that NaHCO3 exposure changed the levels of several physiological parameters associated with the liver, including antioxidant enzymes (SOD, CAT, GSH-Px), MDA, AKP, and CPS. According to the metabolomics study, 90 DEMs are involved in various metabolic pathways such as ketone synthesis and degradation metabolism, glycerophospholipid metabolism, arachidonic acid metabolism, and linoleic acid metabolism. In addition, transcriptomics data analysis showed that a total of 301 DEGs were screened between the control group and the high NaHCO3 concentration group, of which 129 up-regulated genes and 172 down-regulated genes. Overall, NaHCO3 exposure could cause lipid metabolism disorders and induce energy metabolism imbalance in the crucian carp liver. Simultaneously, crucian carp might regulate its saline-alkaline resistance mechanism by enhancing the synthesis of glycerophospholipid metabolism, ketone bodies, and degradation metabolism, at the same time increasing the vitality of antioxidant enzymes (SOD, CAT, GSH-Px) and nonspecific immune enzyme (AKP). Herein, all results will provide new insights into the molecular mechanisms underlying the stress responses and tolerance to saline-alkaline exposure in crucian carp.

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