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Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

文献类型： 外文期刊

作者： Nie, Zhiyi 1 ; Kang, Guijuan 1 ; Yan, Dong 1 ; Qin, Huaide 1 ; Yang, Lifu 4 ; Zeng, Rizhong 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou 571101, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Rubber Trees, Minist Agr & Rural Affairs Peoples Republ China, Haikou 571101, Hainan, Peoples R China

3.Chinese Acad Trop Agr Sci, Key Lab Mat Engn High Performance Nat Rubber, Haikou 571101, Hainan, Peoples R China

4.Chinese Acad Trop Agr Sci, Inst Sci & Tech Informat, Haikou 571101, Hainan, Peoples R China

关键词： Hevea brasiliensis; rubber biosynthesis; farnesyl diphosphate synthase; tapping panel dryness; transcriptome sequencing; iTRAQ; protein-protein interaction; MYB transcription factor

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2023 年 113 卷 3 期

页码：

收录情况： SCI

摘要： Tapping panel dryness (TPD) is a century-old problem that has plagued the natural rubber production of Hevea brasiliensis. TPD may result from self-protective mechanisms of H. brasiliensis in response to stresses such as excessive hormone stimulation and mechanical wounding (bark tapping). It has been hypothesized that TPD impairs rubber biosynthesis; however, the underlying mechanisms remain poorly understood. In the present study, we firstly verified that TPD-affected rubber trees exhibited lower rubber biosynthesis activity and greater rubber molecular weight compared to healthy rubber trees. We then demonstrated that HbFPS1, a key gene of rubber biosynthesis, and its expression products were downregulated in the latex of TPD-affected rubber trees, as revealed by transcriptome sequencing and iTRAQ-based proteome analysis. We further discovered that the farnesyl diphosphate synthase HbFPS1 could be recruited to small rubber particles by HbSRPP1 through protein-protein interactions to catalyze farnesyl diphosphate (FPP) synthesis and facilitate rubber biosynthesis initiation. FPP content in the latex of TPD-affected rubber trees was significantly decreased with the downregulation of HbFPS1, ultimately resulting in abnormal development of rubber particles, decreased rubber biosynthesis activity, and increased rubber molecular weight. Upstream regulator assays indicated that a novel regulator, MYB2-like, may be an important regulator of downregulation of HbFPS1 in the latex of TPD-affected rubber trees. Our findings not only provide new directions for studying the molecular events involved in rubber biosynthesis and TPD syndrome and contribute to rubber management strategies, but also broaden our knowledge of plant isoprenoid metabolism and its regulatory networks.

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