文献类型： 外文期刊

作者： Feng, Zhanyuan ¹ ; Bi, Zhubai ² ; Fu, Dugong ¹ ; Feng, Lihan ³ ; Min, Dangxuan ¹ ; Bi, Chensong ¹ ; Huang, He ¹ ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Key Lab Trop Crops Germplasm Resources Genet Impro, 4 Xueyuan Rd, Haikou 571101, Peoples R China

2.Hainan Acad Agr Sci, Key Lab Vegetable Biol Hainan Prov, Inst Vegetables, 14 Xingdan Rd, Haikou 571100, Peoples R China

3.Chinese Acad Trop Agr Sci, Proving Ground, Nada Town 571737, Danzhou, Peoples R China

关键词： watermelon; tetraploid; morphology; photosynthetic parameters; proteomics; qRT-PCR

期刊名称：BIOENGINEERING-BASEL （ 影响因子：5.046； ）

ISSN：

年卷期： 2022 年 9 卷 12 期

页码：

收录情况： SCI

摘要： Watermelon is an important fruit that is widely distributed around the world. In particular, the production and consumption of watermelon in China ranks first in the world. Watermelon production is severely affected by a variety of biotic and abiotic stresses during cultivation, and polyploidization can promote stress resistance and yield. However, the morphological and physiological characteristics of tetraploid watermelon and the underlying molecular mechanisms are still poorly understood. In this study, we revealed that the leaves, fruits, and seeds of tetraploid watermelon were significantly larger than those of the diploid genotype. Some physiological characteristics, including photosynthetic rate (Pn) and stomatal conductance (Gs), were greater, whereas the intercellular CO2 concentration (Ci) and transpiration rate (Tr) were lower in tetraploid than in diploid watermelon. Two-dimensional gel electrophoresis combined with tandem mass spectrometry (MALDI-TOF/TOF) was performed to compare proteomic changes between tetraploid and diploid watermelon. A total of 21 differentially expressed proteins were identified; excluding the identical proteins, 8 proteins remained. Among them, four proteins were upregulated and four were downregulated in tetraploid versus diploid genotypes. qRT-PCR results showed inconsistencies in gene expression and protein accumulation, indicating a low correlation between gene expression and protein abundance. Generally, this study extends our understanding of the traits and molecular mechanisms of tetraploid watermelon and provides a theoretical basis for watermelon polyploid breeding.