文献类型： 外文期刊

作者： Zhang Kaixin ¹ ; Gu Xuedie ¹ ; Lan Jing ² ; Zhang Yiming ¹ ; Khoso, Pervez Ahmed ³ ; Liu Zhaoyi ¹ ; Li Shu ¹ ;

作者机构： 1.Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China

2.Heilongjiang Acad Agr Sci, Qual & Safety Inst Agr Prod, Harbin 150086, Peoples R China

3.Shaheed Benazir Bhutto Univ Vet & Anim Sci Sakran, Sakrand, Shaheed Benazir, Pakistan

关键词： selenium deficiency; inflammation; MiR-30d-R_1; adrenals; toll-like receptor 4; pig

期刊名称：METALLOMICS （ 影响因子：4.526； 五年影响因子：4.69 ）

ISSN： 1756-5901

年卷期： 2021 年 13 卷 7 期

页码：

收录情况： SCI

摘要： Selenium (Se) is an important trace element to maintain the body's dynamic balance. Lack of Se can cause inflammation. Studies have shown that inflammation often leads to disorders of the hypothalamic-pituitary-adrenal axis, but the mechanism by which Se deficiency causes inflammation of the porcine adrenal glands is still unclear. In order to study the effect of Se deficiency on the adrenal glands of pigs, we obtained Se-deficient pig adrenal glands through a low-Se diet. The results of mass spectrometry showed that the Se content in the Se-deficient group was only one-tenth of the control group. We detected the expression of the toll-like receptor 4 (TLR4) and downstream factors by qRT-PCR and Western blotting, and found that the lack of Se affected the TLR4/NF-kappa B pathway. It is known that miR-155-3p, miR-30d-R_1, and miR-146b have all been verified for targeting relationship with TLR4. We confirmed by qRT-PCR that miR-30d-R_1 decreased most significantly in the Se-deficient pig model. Then we tested 25 selenoproteins and some indicators of oxidative stress. It is confirmed that Se deficiency reduces the antioxidant capacity and induces oxidative stress in pig adrenal tissue. In short, a diet lacking Se induces oxidative stress in pig adrenal tissues and leads to inflammation through the miR-30d-R_1/TLR4 pathway. This study provides a reference for the prevention of adrenal inflammation in pigs from a nutritional point of view.