您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

RID serve as a more appropriate measure than phenol sulfuric acid method for natural water-soluble polysaccharides quantification

文献类型： 外文期刊

作者： Zeng, Canbiao 1 ; Ye, Guangying 2 ; Li, Guochao 1 ; Cao, He 1 ; Wang, Zaihua 2 ; Ji, Shengguo 1 ;

作者机构： 1.Guangdong Pharmaceut Univ, Sch Tradit Chinese Med, Guangzhou 510006, Peoples R China

2.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Prov Key Lab Ornamental Plant Germplasm, Guangzhou, Peoples R China

关键词： Water-soluble polysaccharides; RID detector; Method validation; Molecular weight; Substituent

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：10.723； 五年影响因子：9.964 ）

ISSN： 0144-8617

年卷期： 2022 年 278 卷

页码：

收录情况： SCI

摘要： With structural diversity of water-soluble polysaccharides, their precise quantitative analysis by phenol-sulfuric acid method becomes more difficult and challenging. In this study, the quantification analysis of dextran and glucose in phenol sulfuric acid method was compared in this paper. When the concentration is below 90 mu g/mL, the quantification of glucose is close to theoretical value, however, glucose derivatives have significantly different absorption. Later, quantitative factors of water-soluble polysaccharide in RID measurement were investigated. The optimum temperature was 40 degrees C and linear range was 0.3125-10.0 mg/mL in RID for dextrans (1.0-500 kDa) and glucose derivatives. Method validation studies of the RID method were further performed and compared to conventional phenol sulfuric acid method, which demonstrated that RID measurement is more reliable and satisfactory method. The intervention of water-soluble impurity in RID response should be well control below 6% (w/w). By comparison, the RID measurement could well alleviate drawbacks in phenol-sulfuric acid method.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台