文献类型： 外文期刊

作者： Wei, S ¹ ; Marton, I ² ; Dekel, M ² ; Shalitin, D ² ; Lewinsohn, E ² ; Bravdo, BA ² ; Shoseyov, O ² ;

作者机构： 1.Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel

2.Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel; Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Otto Warburg Ctr Agr Biotechnol, IL-76100 Rehovot, Israel; Jiangsu Acad Agr Sci, Hort Inst, Nanjing 210014, Peoples R China; Agr Res Org, Newe Yaar Res Ctr, Dept Vegetable Crops, IL-30095 Ramat Yishay, Israel

关键词： Compartmentation;Beta-glucosidase;Glycosides;Headspace;Scent;Endoplasmic-reticulum;Linalool synthase;Parasitic wasps;Aroma;compounds;Plant-cells;S-linalool;Identification;Localization;Sequence;Flavor

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

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收录情况： SCI

摘要： Expression of the Aspergillus niger P-glucosidase gene, BGL1, in Nicotiana tabacum plants (cv. Xanthi) had a profound effect on the volatile emissions of intact and crushed leaves. BGL1 was expressed under the control of the cauliflower mosaic virus (CaMV) 35S promoter and targeted to the cytoplasm, cell wall, lytic vacuole (LV), chloroplast or endoplasmic reticulum (ER). Subcellular localization was confirmed by gold immunolabelling, followed by transmission electron microscopy (TEM). Significant beta-glucosidase activity was observed in transgenic plants expressing BGL1 in the cell wall, LV and ER. Compared with controls, all intact transgenic leaves were found to emit increased levels of 2-ethylhexanol, as determined by gas chromatography-mass spectrometry (GC-MS) analysis of the headspace volatiles. Plants expressing BGL1 in the cell wall (Tcw) emitted more trans-caryophyllene than did non-transgenic controls, whereas plants expressing BGL1 in the ER (Ter) and LV (Tvc) emitted more cembrene than did non-transgenic controls. Volatiles released from crushed transgenic leaves and collected with solid-phase microextraction (SPME) polydimethylsiloxane fibre were distinctly enhanced. Significant increases in linalool, nerol, furanoid cis-linalool oxide, 4-methyl-1-pentanol, 6-methyl-hept-5-en-2-ol and 2-ethylhexanol were detected in transgenic plants when compared with wild-type controls. 3-Hydroxyl-beta-ionone levels were increased in crushed Tcw and Ter leaves, but were undetectable in Tvc leaves. The addition of glucoimidazole, a beta-glucosidase inhibitor, abolished the increased emission of these volatiles. These results indicate that the expression of a fungal beta-glucosidase gene in different subcellular compartments has the potential to affect the emission of plant volatiles, and thereby to modify plant-environment communication and aroma of agricultural products.