Combining nitrogen effects and metabolomics to reveal the response mechanisms to nitrogen stress and the potential for nitrogen reduction in maize
文献类型： 外文期刊
作者： Yan-li, Lu 1 ; Gui-pei, Song 1 ; Yu-hong, Wang 1 ; Luo-bin, Wang 1 ; Meng-ze, Xu 1 ; Li-ping, Zhou 2 ; Lei, Wang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Key Lab Plant Nutr & Fertilizer,Minist Agr & Rural, Beijing 100081, Peoples R China
2.Tianjin Acad Agr Sci, Tianjin 300112, Peoples R China
关键词： long-term experiment; nitrogen deficiency; nitrogen excess; metabolites; UPLC-QTOF
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）
ISSN： 2095-3119
年卷期： 2023 年 22 卷 9 期
页码：
收录情况： SCI
摘要： The physiological and metabolic differences in maize under different nitrogen (N) levels are the basis of reasonable N management, which is vital in improving fertilizer utilization and reducing environmental pollution. In this paper, on the premise of defining the N fertilizer efficiency and yield under different long-term N fertilization treatments, the corresponding differential metabolites and their metabolic pathways were analyzed by untargeted metabolomics in maize. N stress, including deficiency and excess, affects the balance of carbon (C) metabolism and N metabolism by regulating C metabolites (sugar alcohols and tricarboxylic acid (TCA) cycle intermediates) and N metabolites (various amino acids and their derivatives). L-alanine, L-phenylalanine, L-histidine, and L-glutamine decreased under N deficiency, and L-valine, proline, and L-histidine increased under N excess. In addition to sugar alcohols and the above amino acids in C and N metabolism, differential secondary metabolites, flavonoids (e.g., kaempferol, luteolin, rutin, and diosmetin), and hormones (e.g., indoleacetic acid, trans-zeatin, and jasmonic acid) were initially considered as indicators for N stress diagnosis under this experimental conditions. This study also indicated that the leaf metabolic levels of N2 (120 kg ha-1 N) and N3 (180 kg ha-1 N) were similar, consistent with the differences in their physiological indexes and yields over 12 years. This study verified the feasibility of reducing N fertilization from 180 kg ha-1 (locally recommended) to 120 kg ha-1 at the metabolic level, which provided a mechanistic basis for reducing N fertilization without reducing yield, further improving the N utilization rate and protecting the ecological environment.
- 相关文献
