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Effect of the Combined Compound Probiotics with Glycyrrhinic Acid on Alleviating Cytotoxicity of IPEC-J2 Cells Induced by Multi-Mycotoxins

文献类型： 外文期刊

作者： Wang, Lijun 1 ; Wang, Xiaomin 1 ; Chang, Juan 1 ; Wang, Ping 1 ; Liu, Chaoqi 1 ; Yuan, Lin 2 ; Yin, Qingqiang 1 ; Zhu, Qun 3 ; Lu, Fushan 4 ;

作者机构： 1.Henan Agr Univ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China

2.Henan Acad Agr Sci, Inst Anim Husb & Vet Med, Zhengzhou 450003, Peoples R China

3.Henan Delin Biol Prod Co Ltd, Xinxiang 453000, Henan, Peoples R China

4.Henan Puai Feed Co Ltd, Zhoukou 466000, Peoples R China

关键词： compound probiotics; glycyrrhinic acid; cytotoxicity alleviation; aflatoxins B1; deoxynivalenol; zearalenone

期刊名称：TOXINS （ 影响因子：5.075； 五年影响因子：5.305 ）

ISSN：

年卷期： 2022 年 14 卷 10 期

页码：

收录情况： SCI

摘要： Aflatoxins B1 (AFB1), deoxynivalenol (DON) and zearalenone (ZEA) are the three most prevalent mycotoxins, whose contamination of food and feed is a severe worldwide problem. In order to alleviate the toxic effects of multi-mycotoxins (AFB1 + DON + ZEA, ADZ) on inflammation and apoptosis in swine jejunal epithelial cells (IPEC-J2), three species of probiotics (Bacillus subtilis, Saccharomyces cerevisiae and Pseudomonas lactis at 1 x 10(5) CFU/mL, respectively) were mixed together to make compound probiotics (CP), which were further combined with 400 mu g/mL of glycyrrhinic acid (GA) to make bioactive materials (CGA). The experiment was divided into four groups, i.e., the control, ADZ, CGA and ADZ + CGA groups. The results showed that ADZ decreased cell viability and induced cytotoxicity, while CGA addition could alleviate ADZ-induced cytotoxicity. Moreover, the mRNA expressions of IL-8, TNF-alpha, NF-Kappa b, Bcl-2, Caspase-3, ZO-1, Occludin, Claudin-1 and ASCT2 genes, and protein expressions of TNF-alpha and Claudin-1 were significantly upregulated in ADZ group; while the mRNA abundances of IL-8, TNF-alpha, NF-Kappa b, Caspase-3, ASCT2 genes, and protein expressions of TNF-alpha and Claudin-1 were significantly downregulated in the ADZ + CGA group. In addition, the protein expressions of COX-2, ZO-1, and ASCT2 were significantly downregulated in the ADZ group, compared with the control group; whereas CGA co-incubation with ADZ could increase these protein expressions to recover to normal levels. This study indicated that CGA could alleviate cytotoxicity, apoptosis and inflammation in ADZ-induced IPEC-J2 cells and protect intestinal cell integrity from ADZ damages.

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