Genome-Wide Identification and Analysis of bHLH Transcription Factors Related to Anthocyanin Biosynthesis in Cymbidium ensifolium
文献类型： 外文期刊
作者： Wang, Meng-Jie 1 ; Ou, Yue 1 ; Li, Zuo 2 ; Zheng, Qing-Dong 1 ; Ke, Yu-Jie 1 ; Lai, Hui-Ping 1 ; Lan, Si-Ren 1 ; Peng, Dong-Hui 1 ; Liu, Zhong-Jian 1 ; Ai, Ye 1 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Landscape Architecture & Art, Key Lab Natl Forestry & Grassland Adm Orchid Conse, Fuzhou 350002, Peoples R China
2.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China
关键词： Cymbidium ensifolium; bHLH transcription factor; anthocyanin biosynthesis; expression analysis
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 24 卷 4 期
页码：
收录情况： SCI
摘要： The basic helix-loop-helix (bHLH) transcription factors are widely distributed across eukaryotic kingdoms and participate in various physiological processes. To date, the bHLH family has been identified and functionally analyzed in many plants. However, systematic identification of bHLH transcription factors has yet to be reported in orchids. Here, 94 bHLH transcription factors were identified from the Cymbidium ensifolium genome and divided into 18 subfamilies. Most CebHLHs contain numerous cis-acting elements associated with abiotic stress responses and phytohormone responses. A total of 19 pairs of duplicated genes were found in the CebHLHs, of which 13 pairs were segmentally duplicated genes and six pairs were tandemly duplicated genes. Expression pattern analysis based on transcriptome data revealed that 84 CebHLHs were differentially expressed in four different color sepals, especially CebHLH13 and CebHLH75 of the S7 subfamily. The expression profiles of CebHLH13 and CebHLH75 in sepals, which are considered potential genes regulating anthocyanin biosynthesis, were confirmed through the qRT-PCR technique. Furthermore, subcellular localization results showed that CebHLH13 and CebHLH75 were located in the nucleus. This research lays a foundation for further exploration of the mechanism of CebHLHs in flower color formation.
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