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Development of a Species-Specific SCAR-PCR Assay for Direct Detection of Sugar Beet Cyst Nematode (Heterodera schachtii) from Infected Roots and Soil Samples

文献类型： 外文期刊

作者： Jiang, Chen 1 ; Zhang, Yingdong 1 ; Yao, Ke 1 ; Abdulsalam, Sulaiman 1 ; Li, Guangkuo 3 ; Gao, Haifeng 3 ; Li, Kemei 4 ; Huang, Wenkun 1 ; Kong, Lingan 1 ; Peng, Deliang 1 ; Peng, Huan 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Plant Protect, Minist Agr, Key Lab Integrated Pest Management Crop Northwest, Urumqi 830091, Peoples R China

4.Xinjiang Agr Univ, Coll Agr, Urumqi 830091, Peoples R China

关键词： sugar beet cyst nematode; Heterodera schachtii; RAPD marker; SCAR-PCR

期刊名称：LIFE-BASEL （ 影响因子：3.251； ）

ISSN：

年卷期： 2021 年 11 卷 12 期

页码：

收录情况： SCI

摘要： Sugar beet cyst nematode (SBCN, Heterodera schachtii) is an important nematode that causes significant yield losses of 25-50% or more in most areas of sugar beet production worldwide. Rapid and accurate identification of this species is essential to support decisions on pest management. However, the difference between H. schachtii and other Heterodera spp. based on morphology is a challenging task. In the present study, a SCAR-PCR assay was developed to identify and differentiate H. schachtii in infected root and soil samples. H. schachtii-species-specific SCAR-PCR primers OPA06-HsF and OPA06-HsR were designed from the randomly amplified polymorphic DNA (RAPD) marker amplified with random primer OPA06. The developed primers specifically amplify a 922-bp fragment from the target populations but did not amplify DNA from non-target cyst nematodes including Heterodera, Globodera, Cactodera, and other related species tested in this study. The sensitivity detection indicated that 5 x 10(-4) of a single cyst, 1/320 of a single second-stage juvenile (J2), or 10 pg of genomic DNA could be detected. The assay accurately identifies the different stages of H. schachtii in sugar beet and oilseed rape roots as well as a single J2 in 10 g of soil. Finally, the SCAR-PCR assay detected H. schachtii in seven samples out of the fifteen field samples. The assay will not only be useful for differentiating H. schachtii from mixed populations of Heterodera spp. but also for effective detection of the species directly from infested samples. The assay also requires no expertise in the taxonomy and morphology of the species but serves to improve the diagnosis of H. schachtii in infested fields.

  • 相关文献

[1]Development of a Species-Specific SCAR-PCR Assay for Direct Detection of Sugar Beet Cyst Nematode (Heterodera schachtii) from Infected Roots and Soil Samples. Jiang, Chen,Zhang, Yingdong,Yao, Ke,Abdulsalam, Sulaiman,Huang, Wenkun,Kong, Lingan,Peng, Deliang,Peng, Huan,Yao, Ke,Li, Guangkuo,Gao, Haifeng,Peng, Huan,Li, Kemei. 2021

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