Natural self-attenuation of pathogenic viruses by deleting the silencing suppressor coding sequence for long-term plant-virus coexistence
文献类型： 外文期刊
作者： Qin, Li 1 ; Wang, Xiaoqing 1 ; Dai, Zhaoji 1 ; Shen, Wentao 2 ; Li, Fangfang 4 ; Wang, Aiming 5 ; Valli, Adrian A. 6 ; Cui, Hongguang 1 ;
作者机构： 1.Hainan Univ, Sch Trop Agr & Forestry, Key Lab Green Prevent & Control Trop Plant Dis & P, Minist Educ, Haikou, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Sanya Res Inst, Natl Key Lab Trop Crops Breeding, Haikou, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Sanya Res Inst, Natl Key Lab Trop Crops Breeding, Sanya, Peoples R China
4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China
5.Agr & Agrifood Canada, London Res & Dev Ctr, London, ON, Canada
6.Spanish Natl Ctr Biotechnol CNB CSIC, Madrid, Spain
期刊名称：PLOS PATHOGENS （ 影响因子：4.9； 五年影响因子：5.4 ）
ISSN： 1553-7366
年卷期： 2025 年 21 卷 6 期
页码：
收录情况： SCI
摘要： Potyviridae is the largest family of plant-infecting RNA viruses. All members of the family (potyvirids) have single-stranded positive-sense RNA genomes, with polyprotein processing as the expression strategy. The 5'-proximal regions of all potyvirids, except bymoviruses, encode two types of leader proteases: the serine protease P1 and the cysteine protease HCPro. However, their arrangement and sequence composition vary greatly among genera or even species. The leader proteases play multiple important roles in different potyvirid-host combinations, including RNA silencing suppression and virus transmission. Here, we report that viruses in the genus Arepavirus, which encode two HCPro leader proteases in tandem (HCPro1-HCPro2), can naturally lose the coding sequences for these two proteins during infection. Notably, this loss is associated with a shift in foliage symptoms from severe necrosis to mild chlorosis or even asymptomatic infections. Further analysis revealed that the deleted region is flanked by two short repeated sequences in the parental isolates, suggesting that recombination during virus replication likely drives this genomic deletion. Reverse genetic approaches confirmed that the loss of leader proteases weakens RNA silencing suppression and other critical functions. A field survey of areca palm trees displaying varied symptom severity identified a transitional stage in which full-length viruses and deletion mutants coexist in the same tree. Based on these findings, we propose a scenario in which full-length isolates drive robust infections and facilitate plant-to-plant transmission, eventually giving rise to leader protease-less variants that mitigate excessive damage to host trees, allowing long-term coexistence with the perennial host. To our knowledge, this is the first report of potyvirid self-attenuation via coding sequence loss.
- 相关文献
作者其他论文 更多>>
-
A telosma mosaic virus-based vector for foreign gene expression and virus-induced gene silencing in passion fruit
作者：Wang, Xiaoqing;Qin, Li;Cui, Hongguang;Dai, Zhaoji;Shen, Wentao;Shen, Wentao;Qiu, Wenping
关键词：
-
A Comprehensive Screening of the Interactors of Areca Palm Necrotic Ringspot Virus (ANRSV) HCPro2 Highlights the Proviral Roles of eIF4A and PGK in Viral Infection
作者：Qin, Li;Liu, Peilan;Dai, Zhaoji;Cui, Hongguang;Shen, Wentao
关键词：
Potyviridae ; leader protease; cysteine protease; eukaryotic initiation factor 4A; phosphoglycerate kinase -
Pearl Bodies as a Potential Source of Secondary Transmission of Papaya Leaf Distortion Mosaic Virus in Carica papaya
作者：Tuo, Decai;Su, Die;Yan, Pu;Xue, Weiqian;Xie, Zhengnan;Zhou, Peng;Kong, Hua;Shen, Wentao;Tuo, Decai;Su, Die;Yan, Pu;Xue, Weiqian;Xie, Zhengnan;Zhou, Peng;Kong, Hua;Shen, Wentao;Su, Die;Xue, Weiqian;Zhao, Mengwei
关键词：infection; papaya leaf distortion mosaic virus; pearl bodies; transmission
-
A Point Mutation in the Pepper Veinal Mottle Virus 6K1 Protein Yields a Stable Attenuated Strain for Engineering Virus Resistance in Pepper Plants
作者：Deng, Changhui;Hu, Weiyao;Dai, Zhaoji;Cui, Hongguang;Deng, Changhui;Hu, Weiyao;Dai, Zhaoji;Cui, Hongguang;Shen, Wentao;Li, Fangfang
关键词：cross protection; pathogen-derived resistance; pepper veinal mottle virus; viral vector; virus resistance
-
Rubisco small subunit (RbCS) is co-opted by potyvirids as the scaffold protein in assembling a complex for viral intercellular movement
作者：Qin, Li;Liu, Hongjun;Liu, Peilan;Jiang, Lu;Dai, Zhaoji;Cui, Hongguang;Qin, Li;Liu, Hongjun;Liu, Peilan;Jiang, Lu;Dai, Zhaoji;Cui, Hongguang;Jiang, Lu;Li, Fangfang;Cheng, Xiaofei;Shen, Wentao;Qiu, Wenping
关键词：
-
Mutating the arginine residue within the FRNK motif of telosma mosaic virus (TelMV) HC-Pro protein attenuates viral infection and confers effective protection against TelMV in passion fruit (Passiflora edulis)
作者：Wang, Linxi;Shi, Wei;Aziz, Asma;Wang, Xiaoqing;Liu, Haobin;Cui, Hongguang;Dai, Zhaoji;Shen, Wentao
关键词：telosma mosaic virus; cross protection; HC-Pro; FRNK; passion fruit; attenuated mutant
-
Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu)
作者：Wang, Yaodi;Wang, Linxi;Liu, Hongjun;Gou, Bei;Hu, Weiyao;Qin, Li;Cui, Hongguang;Dai, Zhaoji;Shen, Wentao;Shen, Wentao;Wang, Aiming
关键词：Areca palm; Areca catechu; Protoplast; Protoplast isolation; PEG-mediated transformation
