Mutating the arginine residue within the FRNK motif of telosma mosaic virus (TelMV) HC-Pro protein attenuates viral infection and confers effective protection against TelMV in passion fruit (Passiflora edulis)
文献类型： 外文期刊
作者： Wang, Linxi 1 ; Shi, Wei 1 ; Aziz, Asma 1 ; Wang, Xiaoqing 1 ; Liu, Haobin 1 ; Shen, Wentao 2 ; Cui, Hongguang 1 ; Dai, Zhaoji 1 ;
作者机构： 1.Hainan Univ, Sch Trop Agr & Forestry, Key Lab Green Prevent & Control Trop Dis & Pests, Minist Educ, 58 Renmin Ave, Haikou 570228, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Sanya Res Inst, Hainan Inst Trop Agr Resources,Hainan Key Lab Prot, Haikou, Peoples R China
关键词： telosma mosaic virus; cross protection; HC-Pro; FRNK; passion fruit; attenuated mutant
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）
ISSN： 1526-498X
年卷期： 2024 年
页码：
收录情况： SCI
摘要： BACKGROUND Telosma mosaic virus (TelMV, Potyvirus, Potyviridae) is an emerging viral pathogen that threatens passion fruit plantations worldwide. However, an efficient strategy for controlling such a virus is not yet available. Cross protection is a phenomenon in which pre-infection of a plant with one mild strain prevents or delays subsequent infection by the same or closely related virus. HC-Pro is the potyviral encoded multifunctional protein involved in several steps of viral infection, including multiplication, movement, transmission and RNA silencing suppression. In this study, we tested whether it is possible to generate attenuated viral strains capable of conferring protection against severe TelMV infection by manipulating the HC-Pro gene. RESULTS By introducing point mutation into the conserved motif FRNK of HC-Pro that is essential for RNA silencing suppression, we have successfully obtained three attenuated mutants of TelMV (R181K, R181D, and R181E, respectively). These attenuated TelMV mutants could systemically infect passion fruit plants without noticeable symptoms. Pre-inoculation of one of these attenuated mutants confers efficient protection against subsequent infection by severe TelMV strain. Moreover, we demonstrated that the HC-Pros harbored by the attenuated mutants exhibit reduced RNA silencing suppression activity in Nicotiana benthamiana leaves. CONCLUSION The attenuated TelMV mutants developed in this study that are suitable for cross protection offer a practical, powerful tool to fight against TelMV for sustainable passion fruit production.
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