Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage
文献类型： 外文期刊
作者： Fu, Zhihui 1 ; Sun, Lin 3 ; Hou, Meiling 4 ; Hao, Junfeng 1 ; Lu, Qiang 5 ; Liu, Tingyu 6 ; Ren, Xiuzhen 6 ; Jia, Yushan 1 ; Wang, ZhiJun 1 ; Ge, Gentu 1 ;
作者机构： 1.Coll Grassland Resources & Environm, Lab Forage Cultivat Proc & High Efficient Utilizat, Minist Agr, Hohhot, Peoples R China
2.Inner Mongolia Agr Univ, Key Lab Grassland Resources, Minist Educ, Hohhot, Peoples R China
3.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China
4.Baicheng Normal Univ, Coll Life Sci, Baicheng, Peoples R China
5.Ningxia Univ, Coll Agr, Yinchuan, Peoples R China
6.Inner Mongolia Minzu Univ, Coll Agr, Tongliao, Peoples R China
关键词： ryegrass silage; bacterial community; metabolomics profiles; SMRT; UHPLC-MS; MS
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： In this study, we analyzed the fermentation quality, microbial community, and metabolome characteristics of ryegrass silage from different harvests (first harvest-AK, second harvest-BK, and third harvest-CK) and analyzed the correlation between fermentative bacteria and metabolites. The bacterial community and metabolomic characteristics were analyzed by single-molecule real-time (SMRT) sequencing and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS), respectively. After 60 days of ensiling, the pH of BK was significantly lower than those of AK and CK, and its lactic acid content was significantly higher than those of AK and CK. Lactiplantibacillus and Enterococcus genera dominate the microbiota of silage obtained from ryegrass harvested at three different harvests. In addition, the BK group had the highest abundance of Lactiplantibacillus plantarum (58.66%), and the CK group had the highest abundance of Enterococcus faecalis (42.88%). The most annotated metabolites among the differential metabolites of different harvests were peptides, and eight amino acids were dominant in the composition of the identified peptides. In the ryegrass silage, arginine, alanine, aspartate, and glutamate biosynthesis had the highest enrichment ratio in the metabolic pathway of KEGG pathway enrichment analysis. Valyl-isoleucine and glutamylvaline were positively correlated with Lactiplantibacillus plantarum. D-Pipecolic acid and L-glutamic acid were positively correlated with Levilactobacillus brevis. L-phenylalanyl-L-proline, 3,4,5-trihydroxy-6-(2-methoxybenzoyloxy) oxane-2-carboxylic acid, and shikimic acid were negatively correlated with Levilactobacillus brevis. In conclusion, this study explains the effects of different harvest frequencies on the fermentation quality, microbial community, and metabolites of ryegrass, and improves our understanding of the ensiling mechanisms associated with different ryegrass harvesting frequencies.
- 相关文献
作者其他论文 更多>>
-
Effect isolated lactic acid bacteria inoculation on the quality, bacterial composition and metabolic characterization of Caragana korshinskii silage
作者：Bai, Baochao;Qiu, Rui;Bao, Jian;Liu, Yichao;Ge, Gentu;Jia, Yushan;Wang, Zhijun;Sun, Lin;Bai, Baochao;Qiu, Rui;Bao, Jian;Liu, Yichao;Ge, Gentu;Jia, Yushan;Bai, Baochao;Qiu, Rui;Bao, Jian;Liu, Yichao;Ge, Gentu;Jia, Yushan;Wang, Zhijun
关键词：Caragana korshinskii; Lactic acid bacteria; Microbial community; Metabolome
-
Effects of selenium enrichment on fermentation characteristics, selenium content and microbial community of alfalfa silage
作者：Sun, Pengbo;Ge, Gentu;Du, Shuai;Liu, Yichao;Yan, Xingquan;Wang, Zhijun;Jia, Yushan;Sun, Pengbo;Ge, Gentu;Du, Shuai;Liu, Yichao;Yan, Xingquan;Wang, Zhijun;Jia, Yushan;Sun, Pengbo;Ge, Gentu;Du, Shuai;Liu, Yichao;Yan, Xingquan;Wang, Zhijun;Jia, Yushan;Sun, Lin;Zhang, Jiawei;Zhang, Yuhan
关键词：Alfalfa; Selenium; Biofermentation; Selenium speciation; In vitro digestion
-
Mechanistic Insights into Nitrite Degradation by Metabolites of L. plantarum A50: An LC-MS-Based Untargeted Metabolomics Analysis
作者：An, Jiangbo;Liu, Mingjian;Dai, Rui;Si, Qiang;Ge, Gentu;Wang, Zhijun;Jia, Yushan;An, Jiangbo;Liu, Mingjian;Dai, Rui;Si, Qiang;Ge, Gentu;Wang, Zhijun;Jia, Yushan;An, Jiangbo;Liu, Mingjian;Dai, Rui;Si, Qiang;Ge, Gentu;Wang, Zhijun;Jia, Yushan;Sun, Lin
关键词：Lactiplantibacillus plantarum; physiological characteristics; metabolic products; nitrites
-
Utilisation of Lactiplantibacillus plantarum and propionic acid to improve silage quality of amaranth before and after wilting: fermentation quality, microbial communities, and their metabolic pathway
作者：Zhao, Muqier;Wang, Zhijun;Sun, Pengbo;Liu, Jingyi;Yan, Yuting;Ge, Gentu;Zhao, Muqier;Wang, Zhijun;Sun, Pengbo;Liu, Jingyi;Yan, Yuting;Ge, Gentu;Bao, Jian
关键词：amaranth silage; fermentation quality; Lactiplantibacillus plantarum; microbial community; moisture content; propionic acid
-
Insights into the microbiome and metabolome assembly during short-term storage of native grass hay
作者：Si, Qiang;Wang, Zhijun;Liu, Mingjian;Liu, Wei;Ge, Gentu;Jia, Yushan;Du, Shuai;Sun, Lin
关键词：Native grass; Hay; Microbial communities; Metabolites; Nutrient composition
-
Biotechnological Processing of Sugarcane Bagasse through Solid-State Fermentation with White Rot Fungi into Nutritionally Rich and Digestible Ruminant Feed
作者：Khan, Nazir Ahmad;Tan, Zhiliang;Liu, Yong;Tang, Shaoxun;Khan, Nazir Ahmad;Khan, Mussayyab;Saeed, Ashmal;Sufyan, Abubakar;Sun, Lin;Wang, Siran;Nazar, Mudasir;Tang, Shaoxun
关键词：sugarcane bagasse; lignocellulosic biomass; fungal treatment; solid-state fermentation; lignin degradation; delignification; digestibility
-
Dynamics of gas and greenhouse gases production during fermentation of barley silage with lactic acid bacteria
作者：Xue, Yanlin;Wu, Nier;Na, Na;Sun, Lin;Qili, Moge;Sun, Juanjuan;Li, Dongyang;Li, E.;Yang, Baozhu
关键词：Bacterial communities; Barley silage; Fermentation weight loss; Gas accumulation phase; Gas reduction phase; Greenhouse gases
