Effects of different harvest frequencies on microbial community and metabolomic properties of annual ryegrass silage
文献类型： 外文期刊
作者： Fu, Zhihui 1 ; Sun, Lin 3 ; Hou, Meiling 4 ; Hao, Junfeng 1 ; Lu, Qiang 5 ; Liu, Tingyu 6 ; Ren, Xiuzhen 6 ; Jia, Yushan 1 ; Wang, ZhiJun 1 ; Ge, Gentu 1 ;
作者机构： 1.Coll Grassland Resources & Environm, Lab Forage Cultivat Proc & High Efficient Utilizat, Minist Agr, Hohhot, Peoples R China
2.Inner Mongolia Agr Univ, Key Lab Grassland Resources, Minist Educ, Hohhot, Peoples R China
3.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot, Peoples R China
4.Baicheng Normal Univ, Coll Life Sci, Baicheng, Peoples R China
5.Ningxia Univ, Coll Agr, Yinchuan, Peoples R China
6.Inner Mongolia Minzu Univ, Coll Agr, Tongliao, Peoples R China
关键词： ryegrass silage; bacterial community; metabolomics profiles; SMRT; UHPLC-MS; MS
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： In this study, we analyzed the fermentation quality, microbial community, and metabolome characteristics of ryegrass silage from different harvests (first harvest-AK, second harvest-BK, and third harvest-CK) and analyzed the correlation between fermentative bacteria and metabolites. The bacterial community and metabolomic characteristics were analyzed by single-molecule real-time (SMRT) sequencing and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS), respectively. After 60 days of ensiling, the pH of BK was significantly lower than those of AK and CK, and its lactic acid content was significantly higher than those of AK and CK. Lactiplantibacillus and Enterococcus genera dominate the microbiota of silage obtained from ryegrass harvested at three different harvests. In addition, the BK group had the highest abundance of Lactiplantibacillus plantarum (58.66%), and the CK group had the highest abundance of Enterococcus faecalis (42.88%). The most annotated metabolites among the differential metabolites of different harvests were peptides, and eight amino acids were dominant in the composition of the identified peptides. In the ryegrass silage, arginine, alanine, aspartate, and glutamate biosynthesis had the highest enrichment ratio in the metabolic pathway of KEGG pathway enrichment analysis. Valyl-isoleucine and glutamylvaline were positively correlated with Lactiplantibacillus plantarum. D-Pipecolic acid and L-glutamic acid were positively correlated with Levilactobacillus brevis. L-phenylalanyl-L-proline, 3,4,5-trihydroxy-6-(2-methoxybenzoyloxy) oxane-2-carboxylic acid, and shikimic acid were negatively correlated with Levilactobacillus brevis. In conclusion, this study explains the effects of different harvest frequencies on the fermentation quality, microbial community, and metabolites of ryegrass, and improves our understanding of the ensiling mechanisms associated with different ryegrass harvesting frequencies.
- 相关文献
作者其他论文 更多>>
-
Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium
作者：Sun, Pengbo;Ge, Gentu;Zhao, Muqier;Hao, Junfeng;Wang, Zhijun;Jia, Yushan;Sun, Lin;Bao, Jian;Zhang, Yuhan;Liu, Guoshun
关键词：Nano-selenium; Alfalfa; Nitrogen metabolism; Transcriptome; Metabolome
-
Effects of Two Bacterial Exopolysaccharides on Microbial Community, Fermentation Characteristics and Aerobic Stability in Oat Silage
作者：Liu, Wei;Wang, Zhijun;Du, Shuai;Ge, Gentu;Jia, Yushan;Liu, Wei;Wang, Zhijun;Du, Shuai;Ge, Gentu;Jia, Yushan;Liu, Wei;Wang, Zhijun;Du, Shuai;Ge, Gentu;Jia, Yushan;Sun, Lin
关键词：bacterial community; dextran; fermentation; levan; oat quality
-
Effects of Moisture Content Gradient on Alfalfa Silage Quality, Odor, and Bacterial Community Revealed by Electronic Nose and GC-MS
作者：Liu, Yichao;Wang, Zhijun;Zhao, Muqier;Hao, Junfeng;Liu, Mingjian;Ge, Gentu;Jia, Yushan;Du, Shuai;Sun, Lin;Zhang, Yuhan
关键词：fermentation quality; odor composition; volatile organic compounds; bacterial diversity; metabolic pathway
-
Foliar Spraying of Nanoselenium Improves the Nutritional Quality of Alfalfa by Recruiting Beneficial Phyllosphere Bacteria and Regulating the Distribution and Translocation of Selenium
作者：Sun, Pengbo;Wang, Zhijun;Ge, Gentu;Liu, Yichao;Yan, Xingquan;Jia, Yushan;Sun, Pengbo;Wang, Zhijun;Ge, Gentu;Liu, Yichao;Yan, Xingquan;Jia, Yushan;Sun, Lin;Bao, Jian;Zhang, Jiawei;Zhang, Yuhan
关键词：nanoselenium; alfalfa; selenium speciation; nutrient quality; phyllosphere bacteria
-
High-moisture alfalfa silage fermentation: a comparative study on the impact of additives including formic acid, Lactobacillus plantarum, cinnamon essential oil, and wood vinegar
作者：Yan, Xingquan;Zhao, Muqier;Liu, Jingyi;Sun, Pengbo;Jia, Yushan;Ge, Gentu;Wang, Zhijun;Yan, Xingquan;Zhao, Muqier;Liu, Jingyi;Sun, Pengbo;Jia, Yushan;Ge, Gentu;Wang, Zhijun;Yan, Xingquan;Zhao, Muqier;Liu, Jingyi;Sun, Pengbo;Jia, Yushan;Ge, Gentu;Wang, Zhijun;Bao, Jian;Liu, Zhuotong;Wang, Mingjiu
关键词：alfalfa; silage; bacterial community; cinnamon essential oil; wood vinegar
-
Addition of hydrothermal biochar significantly alters growth of forage oats and rhizosphere microbiota
作者：Yan, Xingquan;Zhao, Muqier;Liu, Jingyi;Jia, Yushan;Wang, Zhijun;Ge, Gentu;Liu, Zhuotong;Bao, Jian;Wang, Mingjiu
关键词：
-
Mechanistic Insights into Nitrite Degradation by Metabolites of L. plantarum A50: An LC-MS-Based Untargeted Metabolomics Analysis
作者：An, Jiangbo;Liu, Mingjian;Dai, Rui;Si, Qiang;Ge, Gentu;Wang, Zhijun;Jia, Yushan;An, Jiangbo;Liu, Mingjian;Dai, Rui;Si, Qiang;Ge, Gentu;Wang, Zhijun;Jia, Yushan;An, Jiangbo;Liu, Mingjian;Dai, Rui;Si, Qiang;Ge, Gentu;Wang, Zhijun;Jia, Yushan;Sun, Lin
关键词：Lactiplantibacillus plantarum; physiological characteristics; metabolic products; nitrites
