文献类型： 外文期刊

作者： Zhang Guangchen ¹ ; Liu Zimeng ¹ ; Liu Youhong ² ; Noriyuki, Kuya ³ ; Hua Yuchen ¹ ; Shi Hongru ⁴ ; Zhao Weilin ¹ ; Han Y ¹ ;

作者机构： 1.Shenyang Agr Univ, Rice Res Inst, Shenyang 110161, Peoples R China

2.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Tillage, Heilongjiang Prov Key Lab Crop Mol Design & Germp, Haerbin 150086, Peoples R China

3.Natl Agr & Food Res Org, Inst Crop Sci, Ibaraki 3058518, Japan

4.Ctr Seed Ind Dev Liaoning Prov, Shenyang 110034, Peoples R China

5.Okayama Univ, Inst Plant Sci & Resources, Okayama 7100046, Japan

关键词： iTRAQ-based proteomics; direct-seeding; anaerobic germination; weedy rice; differentially abundant protein

期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）

ISSN： 1672-6308

年卷期： 2021 年 28 卷 4 期

页码：

收录情况： SCI

摘要： Direct-seeding of rice has become popular in recent years due to its low cost and convenience, however, hypoxic condition limits seedling establishment. In this study, weedy rice WR04-6 with high germination ability under anaerobic conditions was used as a gene donor, and we successfully improved the seedling establishment rate of rice cultivar Qishanzhan (QSZ) based on selection of a new rice line R42 from the recombinant inbred line population. R42 inherited high anaerobic germination (AG) ability, and was used for isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative proteomic studies with QSZ to further explore the molecular mechanism of AG. A total of 719 differentially abundant proteins (DAPs) were shared by R42 and QSZ responded to AG, and thus defined as common response DAPs. A total of 300 DAPs that responded to AG were only identified from R42, which were defined as tolerance-specific DAPs. The common response and tolerance-specific DAPs had similar biochemical reaction processes and metabolic pathways in response to anoxic stress, however, they involved different proteins. The tolerance-specific DAPs were involved in amino acid metabolism, starch and sucrose metabolism, tricarboxylic acid cycle pathway, ethylene synthesis pathway, cell wall-associated proteins and activity of active oxygen scavenging enzyme. The in silico protein-protein interactions for the top 60 DAPs indicated that tolerance-specific DAPs had relatively independent protein interaction networks in response to an anoxic environment compared with common response DAPs. The results of physiological indicators showed alpha-amylase and superoxide dismutase activities of R42 were significantly increased under anoxic conditions compared with aerobic conditions. Multiple lines of evidence from western blot, physiological analysis and quantitative real-time PCR jointly supported the reliability of proteomics data. In summary, our findings deepened the understanding of the molecular mechanism for the rice response to AG.