Passive eDNA Sampling Characterizes Fish Community Assembly in the Lancang River of Yunnan, China
文献类型： 外文期刊
作者： Ding, Li 1 ; Duan, Xinbin 2 ; Liu, Mingdian 2 ; Chen, Daqing 2 ; Huang, Xiaofeng 3 ; Wang, Dengqiang 2 ; Ma, Baoshan 2 ; Fu, Shijian 1 ; Zhong, Liqiao 2 ;
作者机构： 1.Chongqing Normal Univ, Lab Evolutionary Physiol & Behav, Chongqing Key Lab Conservat & Utilizat Freshwater, Chongqing 401331, Peoples R China
2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Natl Agr Sci Observing & Expt Stn Chongqing, Wuhan 430223, Peoples R China
3.Yangtze Univ, Coll Anim Sci & Technol, Jingzhou 434025, Peoples R China
关键词： environmental DNA; the Lancang River; freshwater fish; passive eDNA samplers
期刊名称：BIOLOGY-BASEL （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN：
年卷期： 2025 年 14 卷 8 期
页码：
收录情况： SCI
摘要： This study aimed to determine the practical efficacy of passive eDNA samplers (PEDS) for monitoring fish diversity in riverine ecosystems. It investigated the utility of environmental DNA (eDNA) in accurately depicting fish composition and diversity within the Lancang River. Environmental DNA technology, particularly PEDS, may be used as a substitute for traditional water filtration techniques. However, its effectiveness in natural water ecosystems remains to be proven. The filter materials included mixed cellulose acetate and nitrate (MCE), nylon (NL), glass fiber (GF), and polyvinyl chloride filter membrane (PVC). This study used four different types of filters, each with identical pore sizes and dimensions but constructed from various materials, to assess eDNA capture under laboratory and field conditions in the water samples. The filter materials included mixed cellulose acetate and nitrate (MCE), nylon (NL), glass fiber (GF), and polyvinyl chloride filter membrane (PVC). Environmental DNA macrobarcoding was used to analyze fish biodiversity and to understand the environmental effects on species distribution. Our study identified 50 fish species inhabiting the Lancang River, with equal representation of exotic and native species. A comparative analysis of four filter-based environmental DNA samplers and traditional environmental DNA sampling methods demonstrated comparable species richness. Redundancy analysis indicated that environmental variables, elevation, electrical conductivity, salinity, and chlorophyll-a significantly influenced the distribution patterns of both non-native and native fish species in the river. This study highlights the significance of eDNA technology in evaluating fish diversity across diverse habitats, thereby establishing a theoretical framework for the sustained monitoring and management of fish biodiversity in protected areas.
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