文献类型： 外文期刊

作者： Sheng, Mengyao ¹ ; Yang, Zhou ¹ ; Yang, Xiaojun ² ; Xu, Jianyi ¹ ; Qiu, Jiguo ¹ ; He, Jian ¹ ; Qiu, Shanlian ³ ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Key Lab Agr Environm Microbiol, Minist Agr, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Customs, Nanjing 210001, Jiangsu, Peoples R China

3.Fujian Acad Agr Sci, Inst Subtrop Agr, Zhangzhou 363005, Fujian, Peoples R China

关键词： Niastella caeni sp. nov.; sludge; polyphasic taxonomy; 16S rRNA gene sequence

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 4 期

页码：

收录情况： SCI

摘要： A Gram-stain-negative, aerobic, non-flagellated and filamentous-shaped bacterium, HX-16-21(T), was isolated from activated sludge. Strain HX-16-21(T) was able to degrade gentisate. protocatechuic acid and p-hydroxybenzoic acid and herbicides quizalofop-p-ethyl and diclofop-methyl. The strain shared 97.2 % 16S rRNA gene sequence similarity to Niastella vici CCTCC AB 2015052(T) and less than 97 % similarities to other type strains. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HX-16-21(T) belonged to the genus Niastella and formed a subclade with N. vici CCTCC AB 2015052(T). The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and six unidentified lipids. The major fatty acids were isoC(15:0') isoC(15:1) G and iso-C-17:0 3-OH. The predominant respiratory quinone was menaquinone 7 (MK-7). The draft genome of strain HX-16-21(T) was 8.1 Mb, and the G+C content was 43.5 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain HX-16-21(T) and N. vici CCTCC AB 2015052(T) were 80.6 and 26.8 %, respectively. Based on both phenotypic and phylogenetic evidence, strain HX-16-21(T) is considered to represent a novel species in the genus Niastella, for which the name Niastella caeni sp. nov. is proposed. The type strain is HX-16-21(T) (=KCTC 72288(T) =ACCC 61580(T)).