A COMPARISON OF SILVER STAINING PROTOCOLS FOR DETECTING DNA IN POLYESTER-BACKED POLYACRYLAMIDE GEL
文献类型: 外文期刊
作者: Qiu, Shanlian 1 ; Chen, Jichen 1 ; Lin, Si 2 ; Lin, Xinjian 1 ;
作者机构: 1.Fujian Acad Agr Sci, Inst Soil & Fertilizer, Fuzhou 350003, Peoples R China
2.Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China
关键词: Backed gel;DNA;Silver staining
期刊名称:BRAZILIAN JOURNAL OF MICROBIOLOGY ( 影响因子:2.476; 五年影响因子:3.391 )
ISSN: 1517-8382
年卷期: 2012 年 43 卷 2 期
页码:
收录情况: SCI
摘要: Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
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