Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia
文献类型： 外文期刊
作者： Cheng, Junwen 1 ; Song, Jiling 2 ; Wei, Hailong 1 ; Wang, Yanbin 1 ; Huang, Xubo 1 ; Liu, Yu 3 ; Lu, Na 2 ; He, Liang 1 ; Lv 1 ;
作者机构： 1.Zhejiang Acad Forestry, Dept Forest Foods, Key Lab Biol & Chem Utilizat Zhejiang Forest Reso, Hangzhou 310023, Peoples R China
2.Hangzhou Acad Agr Sci, Hangzhou 310024, Peoples R China
3.Zhejiang Univ, Coll Life Sci, Inst Biochem, Hangzhou 310058, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China
5.Zhejiang Chinese Med Univ, Hangzhou 310053, Peoples R China
关键词： Sanghuangporus sanghuang; Polysaccharide; Hypoglycemic activity; Structure; AFM
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2020 年 164 卷
页码：
收录情况： SCI
摘要： A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns. Basic monosaccharide composition indicated that SSIPS1 was mainly composed of D-glucose. The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked alpha-D-glucopyranose (Glcp) residues with two branched points at O-6. The two branches were composed of 1,4-linked alpha-D-Glcp terminated with alpha-D-Glcp, 1,4-linked alpha-D-Glcp and 1,4-linked beta-Galp terminated by alpha-D-Glcp. Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy. Further, SSIPS1 exhibited a potential inhibitory activity against a-amylase and alpha-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well. (C) 2020 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
From White to Reddish-Brown: The Anthocyanin Journey in Stropharia rugosoannulata Driven by Auxin and Genetic Regulators
作者：Wang, Mei;Song, Tingting;Jin, Qunli;Zhang, Zuofa;Shen, Yingyue;Lv, Guoying;Fan, Lijun;Feng, Weilin;Qu, Yingmin;Wang, Mengyu;Cai, Weiming;Shen, Meng;Lou, Heqiang
关键词：pileipellis color; SrDRF2; anthocyanin; SrNF-YA; auxin
-
Comparative analysis of the flavonoid components reveals the differences of color and antioxidant activity in two cultivars of Stropharia rugosoannulata
作者：Lv, Guoying;Zhang, Zuofa;Zhao, Ruimeng;Cai, Weiming;Wang, Mengyu;Shen, Meng
关键词：Flavonoid; Stropharia rugosoannulata; Color; Antioxidant activity; Flavonoid; Stropharia rugosoannulata; Color; Antioxidant activity
-
Molecular insights into temperature-driven color variation in Stropharia rugosoannulata mushrooms
作者：Shen, Meng;Wang, Ruisen;Yuan, Ye;Quan, Xinhua;Yao, Xiangtan;Lv, Guoying;Wang, Mengyu
关键词：S. rugosoannulata; Color variation; Transcriptome sequencing; Flavonoid biosynthesis; Transcriptional regulation
-
H2S treatment alleviates postharvest chilling injury in green pepper by regulating antioxidant capacity, osmotic regulation and lipid metabolism to maintain cell membrane integrity
作者：Zhao, Liangyi;Lu, Yixia;Zhao, Yaqin;Bao, Yinqiu;Liu, Yu;Wu, Zhengguo;Zheng, Yonghua;Jin, Peng;Han, Yanchao;Chen, Hangjun
关键词：Green pepper; Chilling injury; Hydrogen sulfide; Antioxidant capacity; Membrane lipid metabolism; S-sulfhydration
-
Integrated metabolomic, transcriptomic, and phytohormone analyses revealed sucrose accumulation mechanism of muskmelon (Cucumis melo) during fruit ripening
作者：Xu, Huan-Wen;Wang, Jiang-Yan;Sun, Ji;Zhang, Sheng-Mei;Shui, De-Ju;Wang, Jiang-Yan;Feng, Xi-Bo;Liu, Yu;Liu, Kun
关键词：Muskmelon; RNA-Seq; Plant hormone; Metabolome; Regulatory network
-
A novel glucan from Sparassis latifolia: purification, structural characterization and protective effect on gastric mucosal cells against ethanol-induced damage
作者：Wang, Mengyu;Lv, Guoying;Zhao, Ruimeng;Cai, Weiming;Zhang, Zuofa
关键词：
Sparassis latifolia ; Polysaccharide; Structural characterization; Gastroprotective effect; Gastric mucosal cells; Underlying mechanisms -
Protective Effects of Polygonatum sibiricum Polysaccharides Against Type 2 Diabetic Mice Induced by High-Fat Diet and Low-Dose Streptozotocin
作者：Li, Qingxiangzi;He, Liang;Li, Qingxiangzi;Cheng, Jufen;He, Liang;Sun, Yangyang;Li, Rui
关键词：
Polygonatum sibiricum polysaccharide; diabetes; glycolipid metabolism; gut microbiota
