Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia
文献类型： 外文期刊
作者： Cheng, Junwen 1 ; Song, Jiling 2 ; Wei, Hailong 1 ; Wang, Yanbin 1 ; Huang, Xubo 1 ; Liu, Yu 3 ; Lu, Na 2 ; He, Liang 1 ; Lv 1 ;
作者机构： 1.Zhejiang Acad Forestry, Dept Forest Foods, Key Lab Biol & Chem Utilizat Zhejiang Forest Reso, Hangzhou 310023, Peoples R China
2.Hangzhou Acad Agr Sci, Hangzhou 310024, Peoples R China
3.Zhejiang Univ, Coll Life Sci, Inst Biochem, Hangzhou 310058, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China
5.Zhejiang Chinese Med Univ, Hangzhou 310053, Peoples R China
关键词： Sanghuangporus sanghuang; Polysaccharide; Hypoglycemic activity; Structure; AFM
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2020 年 164 卷
页码：
收录情况： SCI
摘要： A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns. Basic monosaccharide composition indicated that SSIPS1 was mainly composed of D-glucose. The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked alpha-D-glucopyranose (Glcp) residues with two branched points at O-6. The two branches were composed of 1,4-linked alpha-D-Glcp terminated with alpha-D-Glcp, 1,4-linked alpha-D-Glcp and 1,4-linked beta-Galp terminated by alpha-D-Glcp. Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy. Further, SSIPS1 exhibited a potential inhibitory activity against a-amylase and alpha-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well. (C) 2020 Elsevier B.V. All rights reserved.
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