Conformational properties and biological activities of alpha-D-mannan from Sanghuangporus sanghuang in liquid culture
文献类型： 外文期刊
作者： Cheng, Junwen 1 ; Song, Jiling 2 ; Liu, Yu 3 ; Lu, Na 2 ; Wang, Yanbin 1 ; Hu, Chuanjiu 1 ; He, Liang 1 ; Wei, Hailong 1 ; L 1 ;
作者机构： 1.Zhejiang Acad Forestry, Dept Forest Foods, Key Lab Biol & Chem Utilizat Zhejiang Forest Reso, Hangzhou 310023, Peoples R China
2.Hangzhou Acad Agr Sci, Hangzhou 310024, Peoples R China
3.Zhejiang Univ, Coll Life Sci, Inst Biochem, Hangzhou 310058, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Peoples R China
关键词： Sanghuangporus sanghuang; Mannan; Conformation
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2020 年 164 卷
页码：
收录情况： SCI
摘要： Sanghuang is a well-known edible and medicinal fungus consumed in Asia for centuries. In the present study, a novel exopolysaccharide named as mannan (SSEPS2) was isolated from the liquid culture broth of Sanghuangporus sanghuang by using ethanol precipitation, anion-exchange chromatography, and size-exclusion chromatography, which was exclusively composed of D-mannose with the molecular weight 9.43 x 10(4) g/mol. The structural analysis suggested that SSEPS2 consisted of 1,3-linked and 1,2-linked alpha-D-mannopyranose (Manp), with substitution at O-6 of 1,2-linked alpha-D-Manp by 1,6-linked alpha-D-Manp residues and terminal alpha-D-Manp residues. The exponent of a (0.58) indicated that SSEPS2 existed in a flexible chain conformation, with a coil-like structure in 0.1 M NaNO3 at 25 degrees C, which was further confirmed by the atomic force microscopy images and a three-dimensional molecular model. It exhibited potential antitumor activity against the growth of HepG2 (human hepatoma) and MCF-7 cell lines in vitro. The IC50 values for inhibiting HepG-2 cells and MCF-7 cells proliferation were 365 and 340 mu g/mL, which showed its potential application in the development of anticancer therapeutics. (C) 2020 Elsevier B.V. All rights reserved.
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