Establishment and application of a microsatellite-based parentage identification method for Oreochromis niloticus, O. aureus, and O. niloticus x O. aureus
文献类型： 外文期刊
作者： Liu, Zhigang 1 ; Cao, Jianmeng 1 ; Lu, Maixin 1 ; Gao, Fengying 1 ; Ke, Xiaoli 1 ; Wang, Miao 1 ; Yi, Mengmeng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Guangzhou, Peoples R China
关键词： microsatellite markers; mixed population; Oreochromis aureus; Oreochromis niloticus; Oreochromis niloticus × Oreochromis aureus; parentage identification
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN： 0175-8659
年卷期： 2021 年 37 卷 1 期
页码：
收录情况： SCI
摘要： In genetic fish breeding research, clear pedigree information is of great significance for breeding and parental management. In order to establish a stable, highly accurate, and widely applicable parentage identification method for tilapia, 13 highly polymorphic microsatellite loci within populations of Oreochromis niloticus, O. aureus, O. niloticus x O. aureus, and their mixed population were screened. Four groups of fluorescent-labeled multiple capillary electrophoresis were established for allelic genotyping. The assignment success rate reached 100% when 7, 9, 8 and 12 loci were used in the population of O. niloticus, O. aureus, O. niloticus x O. aureus, and their mixed population, respectively. All 175 progeny individuals of "Yuemin No. 1" tilapia were exclusively assigned to their parental pairs when the 12 loci for the mixed population were used. This study established a fluorescent-labeled microsatellite-based parentage assignment method for O. niloticus, O. aureus, O. niloticus x O. aureus, and their mixed population with high identification accuracy and efficiency, which lays a foundation for pedigree information and population genetic management in tilapia breeding.
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