文献类型： 外文期刊

作者： Xu Jun-Yi ¹ ; Cao Ji-Juan ² ; Zheng Qiu-Yue ² ; Yang Li-Li ¹ ; Wang Yong ³ ; Zou Ming-Qiang ⁴ ;

作者机构： 1.Technol Ctr Dalian Customs Dist, Dalian 116001, Peoples R China

2.Dalian Minzu Univ, Minist Educ, Key Lab Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China

3.Tianjin Acad Agr Sci, Tianjin 300192, Peoples R China

4.Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China

5.China Inspect Lab Technol Co Ltd, Beijing 100123, Peoples R China

关键词： Taqman microfluidic chip; Transgenic maize; Event identification; Multi-events parallel detection

期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY （ 影响因子：1.134； 五年影响因子：0.909 ）

ISSN： 0253-3820

年卷期： 2020 年 48 卷 11 期

页码：

收录情况： SCI

摘要： Taqman microfluidic chip technology was applied to the high-throughput identification and detection of 17 transgenic maize events on a real-time fluorescent PCR platform. In one PCR amplification process , 2 maize endogenous genes (hmgA gene , adh1 gene) and 1 loading control gene (18S gene) were used as internal reference, which could simultaneously complete totally 17 events of transgenic maize single-well single-plex amplification parallel detection, including TC1507 , NK603 , MON87640 , MON863 , MON810 , MIR162 , GA21 , DAS40278 , BT176 , BT11 , 98140 , 59122 , 3272 , MON89034 , MIR604 , MON88017 and T25 , and the limit of detection reached 10 -20 copies. The method showed high specificity and sensitivity , and the detection result obtained by this method was completely consistent with that of the single real-time fluorescent PCR method of " gold standard" . The method had the advantages of parallel detection of multiple samples and multiple targets , and provided an efficient and rapid approach 14 the identification of mixture of multiple events of genetically modified products. With this method , 9 batches of transgenic maize samples were detected from 16 batches of imported practical maize, containing 1-8 events. This method could be used for high-throughput detection of mixed transgenic multi-events in imported agricultural products at the port.