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Impacts of lmo2672 Gene Deficiency on Pathogenicity of Listeria monocytogenes

文献类型： 外文期刊

作者： Li, Jie 1 ; Zhang, Xingxing 2 ; Qiao, Mengfan 1 ; Li, Jing 1 ; Li, Yan 1 ; Wang, Xiaoting 1 ; Zhang, Guowu 1 ; Zhang, Kai 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Xinjiang, Peoples R China

3.Cent South Univ, Sch Biol Engn, Changsha 410012, Hunan, Peoples R China

4.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China

关键词： Listeria monocytogenes; lmo2672; Pathogenicity; AraC family

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2020 年 24 卷 4 期

页码：

收录情况： SCI

摘要： Listeria monocytogenes (LM) is an important food borne pathogen, which is severely harmful to human and animals In order to explore the roles of lmo2672 on pathogenicity of LM, a Delta lmo2672 deletion strain was generated using homologous recombination technique, and then the virulence of Delta lmo2672 mutant was analyzed in macrophage cells and mice. The transcription of virulence-related genes was also determined by real-time RT-PCR. Besides, the interaction between lmo2672 and the upstream DNA sequence of prfA gene was investigated by electrophoretic mobility shift assay (EMSA). The results showed that 1) the hemolytic ability of the Delta lmo2672 mutant was significantly weaker than that of wild-type strain LM EGDe (P < 0.05); 2) the invasion and intracellular proliferation in macrophage cells RAW264.7 of LM-Delta lmo2672 were significantly decreased (P < 0.05); 3) the 50% lethal dose (LD 50 ) to mice for the Delta lmo2672 mutant was significantly higher than wild-type strain (P < 0.05); 4) the bacterial loads of the Delta lmo2672 strain in mouse liver and spleen were significantly less than that of wild-type strain (P < 0.05); 5) the transcription levels of virulence-related genes prfA, actA and hly in Delta lmo2672 mutant declined significantly (P < 0.05) as compared with LM EGD-e. Furthermore, EMSA confirmed that there existed the interaction between lmo2672 and the upstream DNA sequence of prfA gene. These findings suggested that AraC family transcription regulator lmo2672 is involved in pathogenicity of LM, which provided an insight into the biological function of lmo2672 in LM virulent regulation. (C) 2020 Friends Science Publishers

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