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Assessment of Yangtze sturgeon as recipient for the production of American paddlefish gametes through spermatogonia transplantation

文献类型： 外文期刊

作者： Ye, Huan 1 ; Takeuchi, Yutaka 2 ; Wu, Mengbin 1 ; Yue, Huamei 1 ; Ruan, Rui 1 ; Du, Hao 1 ; Zhou, Congli 1 ; Xiang, Hao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs, Wuhan 430223, Peoples R China

2.Kanazawa Univ, Fac Biol Sci & Technol, Noto Ctr Fisheries Sci & Technol, Kanazawa, Ishikawa 9270552, Japan

关键词： Spermatogonia; Xenogeneic transplantation; Acipenseriformes; Endangered species

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2020 年 158 卷

页码：

收录情况： SCI

摘要： The Chinese paddlefish (Psephurus gladius), one of the world's largest freshwater fish, was last seen alive in 2003; they are presumed now to be extinct. In fish, germ cell transplantation is currently known as one of the most powerful assisted reproductive technologies for the conservation of endangered species. In the event that a Chinese paddlefish is unexpectedly caught in the near future, we aimed to develop an experimental strategy to produce paddlefish gametes in the gonads of surrogate sturgeon. Spermatogonia were collected from the testes of 2.5-year-old immature male American paddlefish (Polyodon spathula), the species most closely related to the Chinese paddlefish, by Percoll gradient centrifugation, and transplanted into the peritoneal cavity of Yangtze sturgeon (Acipenser dabryanus) larvae at 7-8 days post-hatch. At two months post-transplantation, donor-derived spermatogonia had efficiently colonized in the recipient gonads and proliferated. A PCR analysis developed to detect xenogenic donor-derived mtDNA sequences in recipient gonads revealed that American paddlefish germ cells survived for at least seven months after transplantation in the gonads of Yangtze sturgeon recipients. These results show that the somatic microenvironment of Yangtze sturgeon gonads was able to support the colonization, proliferation, and survival of xenogeneic germ cells from a different taxonomic family. This study provides key information that could lead to future restoration of Chinese paddlefish using germ cell transplantation. (C) 2020 Published by Elsevier Inc.

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