Rice Stripe Virus Coat Protein-Mediated Virus Resistance Is Associated With RNA Silencing in Arabidopsis
文献类型： 外文期刊
作者： Sun, Feng 1 ; Hu, Peng 1 ; Wang, Wei 1 ; Lan, Ying 1 ; Du, Linlin 1 ; Zhou, Yijun 1 ; Zhou, Tong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Minist Sci & Technol, Inst Plant Protect, State Key Lab Cultivat Base,Jiangsu Key Lab Food, Nanjing, Peoples R China
2.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China
3.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China
关键词： Rice stripe virus; CP-mediated resistance; RNA silencing; deep sequence; dcl2; 3; 4
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： Rice stripe virus (RSV) causes rice stripe disease, which is one of the most serious rice diseases in eastern Asian countries. It has been shown that overexpression of RSV coat protein (CP) in rice plants enhances resistance against virus infection. However, the detailed mechanism underlying RSV CP-mediated virus resistance remains to be determined. In this study, we show that both translatable and non-translatable RSV CP transgenic Arabidopsis plants exhibited immunity to virus infection. By using deep sequencing analysis, transgene-derived small interfering RNAs (t-siRNAs) from non-translatable CP transgenic plants and virus-derived small interfering RNAs (vsiRNAs) mapping in the CP region from RSV-infected wild-type plants showed similar sequence distribution patterns, except for a significant increase in the abundance of t-siRNA reads compared with that of CP-derived vsiRNAs. To further test the correlation of t-siRNAs with RSV immunity, we developed RSV CP transgenic Arabidopsis plants in an siRNA-deficient dcl2/3/4 mutant background, and these CP transgenic plants showed the same sensitivity to RSV infection as non-transgenic plants. Together, our data indicate that the expression of RSV CP protein from a transgene is not a prerequisite for virus resistance and RSV CP-mediated resistance is mostly associated with the RNA silencing mechanism in Arabidopsis plants.
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