Rice Stripe Virus Coat Protein-Mediated Virus Resistance Is Associated With RNA Silencing in Arabidopsis
文献类型： 外文期刊
作者： Sun, Feng 1 ; Hu, Peng 1 ; Wang, Wei 1 ; Lan, Ying 1 ; Du, Linlin 1 ; Zhou, Yijun 1 ; Zhou, Tong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Minist Sci & Technol, Inst Plant Protect, State Key Lab Cultivat Base,Jiangsu Key Lab Food, Nanjing, Peoples R China
2.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China
3.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China
关键词： Rice stripe virus; CP-mediated resistance; RNA silencing; deep sequence; dcl2; 3; 4
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： Rice stripe virus (RSV) causes rice stripe disease, which is one of the most serious rice diseases in eastern Asian countries. It has been shown that overexpression of RSV coat protein (CP) in rice plants enhances resistance against virus infection. However, the detailed mechanism underlying RSV CP-mediated virus resistance remains to be determined. In this study, we show that both translatable and non-translatable RSV CP transgenic Arabidopsis plants exhibited immunity to virus infection. By using deep sequencing analysis, transgene-derived small interfering RNAs (t-siRNAs) from non-translatable CP transgenic plants and virus-derived small interfering RNAs (vsiRNAs) mapping in the CP region from RSV-infected wild-type plants showed similar sequence distribution patterns, except for a significant increase in the abundance of t-siRNA reads compared with that of CP-derived vsiRNAs. To further test the correlation of t-siRNAs with RSV immunity, we developed RSV CP transgenic Arabidopsis plants in an siRNA-deficient dcl2/3/4 mutant background, and these CP transgenic plants showed the same sensitivity to RSV infection as non-transgenic plants. Together, our data indicate that the expression of RSV CP protein from a transgene is not a prerequisite for virus resistance and RSV CP-mediated resistance is mostly associated with the RNA silencing mechanism in Arabidopsis plants.
- 相关文献
作者其他论文 更多>>
-
Comparative transcriptome profiling reveals the key genes and molecular mechanisms involved in rice under blast infection
作者：Li, Gang;Wang, Jian;Cheng, Baoshan;Wang, Di;Gao, Hao;Xu, Weijun;Wang, Wei;Gao, Qingsong;Zhang, Wenxia;Ji, Jianhui;Li, Bianhao;Zhang, Guoliang;Qi, Zhongqiang;Liu, Yongfeng
关键词：Rice; Blast; Transcriptome; Disease resistance; Hormones; Biochemical indicators
-
Disruption of hindgut microbiome homeostasis promotes postpartum energy metabolism disorders in dairy ruminants by inhibiting acetate-mediated hepatic AMPK-PPARA axis
作者：Wang, Shuo;Kong, Fanlin;Zhang, Xinyue;Dai, Dongwen;Li, Chen;Cao, Zhijun;Wang, Yajing;Wang, Wei;Li, Shengli;Kong, Fanlin
关键词：Energy metabolism disorders; Transition period; Microbiome; Cohort study; Multi-omics analysis; Microbial-host interaction; Cow; Ketosis
-
Loss-of-Function of Two PD-Associated Proteins Confers Resistance to Rice Stripe Virus
作者：Lu, Hong;Ge, Panpan;Ma, Yonghuan;Cui, Feng;Ge, Panpan;Ma, Yonghuan;Cui, Feng;Lin, Feng;Zhou, Tong;Jing, Dedao
关键词：flotillin; importin alpha; PD-associated protein; plasmodesmata; rice; rice stripe virus
-
Protease activity of NIa-Pro determines systemic pathogenicity of clover yellow vein virus
作者：Zhuang, Xinjian;Zhang, Wenyi;Xu, Shuqi;Yang, Wenxuan;Yin, Jinlong;Zhou, Tong;Xu, Kai;Yang, Wenxuan;Zhou, Tong;Kundu, Jiban K.
关键词：CIYVV; Infectious clone; NIa-pro; PVX; Nicotiana benthamiana
-
Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector
作者：Du, Linlin;Li, Tao;Wang, Yunyue;Du, Linlin;Li, Tao;Li, Chenyang;Lan, Ying;Lin, Feng;Zhou, Yijun;Zhou, Tong;Guo, Wei;Xu, Kai;Kundu, Jiban Kumar;Du, Linlin;Zhou, Tong
关键词：Mixed sample test; RSV; SRBSDV; Sentinel surveillance; SBPH
-
RNA interference-based dsRNA application confers prolonged protection against rice blast and viral diseases, offering a scalable solution for enhanced crop disease management
作者：Chen, Pan;Ding, Shaochen;Du, Ruonan;Hu, Xiaoxiao;Zhang, Han;Yu, Hanxi;Xu, Le;Niu, Dongdong;Liu, Hongxia;Zhao, Hongwei;Lan, Ying;Li, Chenyang;Lin, Feng;Du, Linlin;Zhou, Tong;Umida, Isashova;Ray, Rumiana;Liu, Tong;Liang, You
关键词：gene silence;
MoPth11 ; plant protection; rice blast; rice stripe disease; RNAi; RSV; SIGS; small RNA -
Experimental study on the impact outcomes of droplets on rice foliage: influence of liquid properties and leaf angles
作者：Wang, Shilin;Wang, Wei;Lu, Daipeng;Li, Xue;Xu, Dejin;Xu, Guangchun;Nuyttens, David;Lu, Daipeng
关键词：droplet impaction; rice leaves; wetting behavior; adhesion; bounce; shatter
