Hypoxia-induced miR-92a regulates p53 signaling pathway and apoptosis by targeting calcium-sensing receptor in genetically improved farmed tilapia (Oreochromis niloticus)
文献类型： 外文期刊
作者： Qiang, Jun 1 ; He, Jie 1 ; Tao, Yi-Fan 1 ; Bao, Jin-Wen 1 ; Zhu, Jun-Hao 1 ; Xu, Pao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Freshwater Fisheries Res Ctr, Wuxi, Jiangsu, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2020 年 15 卷 11 期
页码：
收录情况： SCI
摘要： miR-92a miRNAs are immune molecules that regulate apoptosis (programmed cell death) during the immune response. Apoptosis helps to maintain the dynamic balance in tissues of fish under hypoxia stress. The aim of this study was to explore the role and potential mechanisms of miR-92a in the liver of tilapia under hypoxia stress. We first confirmed that CaSR (encoding a calcium-sensing receptor) is a target gene of miR-92a in genetically improved farmed tilapia (GIFT) using luciferase reporter gene assays. In GIFT under hypoxia stress, miR-92a was up-regulated and CaSR was down-regulated in a time-dependent manner. Knocked-down CaSR expression led to inhibited expression of p53, TP53INP1, and caspase-3/8, reduced the proportion of apoptotic hepatocytes, and decreased the activity of calcium ions induced by hypoxia in hepatocytes. GIFT injected in the tail vein with an miR-92a agomir showed up-regulation of miR-92a and down-regulation of CaSR, p53, TP53INP1, and caspase-3/8 genes in the liver, resulting in lower serum aspartate aminotransferase and alanine aminotransferase activities under hypoxia stress. These findings suggest that stimulation of miR-92a interferes with hypoxia-induced apoptosis in hepatocytes of GIFT by targeting CaSR, thereby alleviating liver damage. These results provide new insights into the adaptation mechanisms of GIFT to hypoxia stress.
- 相关文献
作者其他论文 更多>>
-
Integration of transcriptome, histopathology, and physiological indicators reveals regulatory mechanisms of largemouth bass ( Micropterus salmoides) in response to carbonate alkalinity stress
作者：Hua, Jixiang;Xi, Bingwen;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Li, Yan;Dong, Yalun;Jiang, Bingjie;Xi, Bingwen;Qiang, Jun
关键词：Micropterus salmoides; Carbonate alkalinity stress; Tissue damage; Serum biological chemistry; RNA-seq
-
Regulatory patterns of testis and ovary maturation in largemouth bass (Micropterus salmoides) revealed by transcriptome profiling
作者：Wang, Qingchun;Lu, Siqi;Hua, Jixiang;Tao, Yifan;Qiang, Jun;Wang, Qingchun;Zhuge, Yan;Chen, Wenhua;Duan, Xuejun
关键词：Micropterus salmoides; Transcriptome; Gonad maturation; Growth dimorphism; FISH
-
Biochemical indices, histological observations and transcriptome sequencing reveal the response mechanism of largemouth bass (Micropterus salmoides) to transport stress
作者：Duan, Xue Jun;Tao, Yi Fan;Hua, Jixiang;Qiang, Jun;Wang, Qing Chun
关键词：Micropterus salmoides; Transport stress; Intestinal tissue; Oxidative stress; Apoptosis; Transcriptome
-
Muscle quality enhancement of largemouth bass (Micropterus salmoides) by alkaline water cultured environment: A comprehensive evaluation of physical features, nutrient composition, and flavor characteristics
作者：Hua, Jixiang;Jiao, Xidong;Wang, Wen;Xi, Bingwen;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Dong, Yalun;Wang, Wen;Xi, Bingwen;Qiang, Jun;Jiao, Xidong;Fan, Daming
关键词：Micropterus salmoides; Alkaline water culture; Muscle quality; Nutrient composition; GC-IMS; Volatile compounds
-
Gut Microbiota Contribute to Heterosis for Growth Trait and Muscle Nutrient Composition in Hybrid Largemouth Bass (Micropterus salmoides)
作者：Hua, Jixiang;Tao, Yifan;Sun, Hui;Liu, Kai;He, Jie;Qiang, Jun;Wang, Qingchun;Zhuge, Yan;Chen, Wenhua;Tao, Yifan;Lu, Siqi;Liu, Kai;Qiang, Jun
关键词：hybridization; microbiota taxa; amino acid; fatty acid; non-additive
-
Genetic Structure, Selective Signatures, and Single Nucleotide Polymorphism Fingerprints of Blue Tilapia (Oreochromis aureus), Nile Tilapia Oreochromis niloticus), and Red Tilapia (Oreochromis spp.), as Determined by Whole-Genome Resequencing
作者：Hua, Jixiang;Tao, Yifan;Wang, Qingchun;Sun, Hui;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Dong, Yalun;Qiang, Jun
关键词：tilapia; whole-genome resequencing; genetic diversity and structure; selective features; fingerprinting
-
Artificial intelligence in smart seafood safety across the supply chains: Recent advances and future prospects
作者：Jiao, Xidong;Zhu, Jinlin;Ye, Weijian;Zou, Hao;Yan, Bowen;Zhang, Nana;Zhang, Hao;Fan, Daming;Zhu, Jinlin;Ye, Weijian;Zou, Hao;Yan, Bowen;Zhang, Nana;Zhang, Hao;Jiao, Xidong;Qiang, Jun;Tao, Yifan;Jiao, Xidong;Qiang, Jun;Tao, Yifan;Zhang, Dachuan;Zhu, Jinlin;Fan, Daming;Fan, Daming
关键词：Artificial intelligence; Seafood safety; Aquaculture; Freshness monitoring; Seafood adulteration
