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Effects of ammonia exposure on antioxidant function, immune response and NF-kappa B pathway in Chinese Strip-necked Turtle (Mauremys sinensis)

文献类型： 外文期刊

作者： Liang, Lingyue 1 ; Huang, Zubin 1 ; Li, Na 1 ; Wang, Dongmei 2 ; Ding, Li 1 ; Shi, Haitao 1 ; Hong, Meiling 1 ;

作者机构： 1.Hainan Normal Univ, Coll Life Sci, Key Lab Ecol Trop Isl, Key Lab Trop Anim & Plant Ecol Hainan Prov,Minist, Haikou 571158, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Trop Biol Technol Res Inst, Haikou 571101, Hainan, Peoples R China

关键词： Mauremyssinensis; Ammonia stress; Antioxidation; Nrf2 signaling; Cytokines

期刊名称：AQUATIC TOXICOLOGY （ 影响因子：4.964； 五年影响因子：5.071 ）

ISSN： 0166-445X

年卷期： 2020 年 229 卷

页码：

收录情况： SCI

摘要： As one of the main toxic substances in aquaculture water, ammonia causes seriously physiological harm to aquatic animals. In order to investigate the effects of ammonia exposure on the antioxidant defense, immune response, and NF-KB signaling pathway in Chinese Strip-necked Turtle (Mauremys sinensis), we designed two experimental groups (control and 6.45 mM ammonia), and sampled at 6 h, 24 h, 48 h, re 24 h (recover 24 h), and re 48 h. The results showed that the blood ammonia (BA) content was significantly increased when the turtles were subjected to ammonia, and the activities of cholinesterase (CHE) and aspartate aminotransferase (AST) in the serum also showed a significant upward trend. The malondialdehyde (MDA) content continuously increased during ammonia exposure, and more than doubled at 48 h compared with the control group. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and their corresponding relative mRNA expression levels in the liver during ammonia exposure were obviously increased when compared to the control group, but most decreased to the normal levels at re 48 h. In addition, the relative mRNA and protein expression levels of NF-E2 related factor 2 (Nrf2) showed similar up-regulation patterns to antioxidase during ammonia exposed periods; whereas kelch-like ECH-binding protein 1 (Keap1), as Nrf2 negative regulator, showed opposite patterns. Moreover, the relative mRNA expression levels of heat shock proteins (HSP70, HSP90) significantly elevated upon the exposure of ammonia. Furthermore, ammonia increased the relative mRNA and protein expression levels of p50 and p65 at different exposed times. The reative mRNA expression levels of immune cytokines (BAFF and IL-6) were upregulated during ammonia exposured time, while there was a decline but did not return to normal levels, in the recovery periods. Taken together, these results indicated that anti oxidation, immunity, and NF-KB signaling played a certain protective role for Mauremys sinensis under ammonia exposure. Our results will be helpful to understand the mechanism of aquatic toxicology induced by ammonia in turtles.

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