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Identification of candidate genes controlling chilling tolerance of rice in the cold region at the booting stage by BSA-Seq and RNA-Seq

文献类型： 外文期刊

作者： Guo, Zhenhua 1 ; Cai, Lijun 3 ; Chen, Zhiqiang 2 ; Wang, Ruiying 1 ; Zhang, Lanming 1 ; Guan, Shiwu 1 ; Zhang, Shuhua; 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Rice Res Inst, Jiamusi 154026, Peoples R China

2.South China Agr Univ, Natl Engn Res Ctr Plant Space Breeding, Guangzhou 510642, Peoples R China

3.Heilongjiang Acad Agr Sci, Jiamusi Branch, Jiamusi 154007, Peoples R China

关键词： Oryza sativa; chilling tolerance; candidate gene; BSA-Seq; RNA-Seq

期刊名称：ROYAL SOCIETY OPEN SCIENCE （ 影响因子：2.963； 五年影响因子：3.44 ）

ISSN： 2054-5703

年卷期： 2020 年 7 卷 11 期

页码：

收录情况： SCI

摘要： Rice is sensitive to low temperatures, specifically at the booting stage. Chilling tolerance of rice is a quantitative trait loci that is governed by multiple genes, and thus, its precise identification through the conventional methods is an arduous task. In this study, we investigated the candidate genes related to chilling tolerance at the booting stage of rice. The F2 population was derived from Longjing25 (chilling-tolerant) and Longjing11 (chilling-sensitive) cross. Two bulked segregant analysis pools were constructed. A 0.82 Mb region containing 98 annotated genes on chromosomes 6 and 9 was recognized as the candidate region associated with chilling tolerance of rice at the booting stage. Transcriptomic analysis of Longjing25 and Longjing11 revealed 50 differentially expressed genes (DEGs) on the candidate intervals. KEGG pathway enrichment analysis of DEGs was performed. Nine pathways were found to be enriched, which contained 10 DEGs. A total of four genes had different expression patterns or levels between Longjing25 and Longjing11. Four out of the 10 DEGs were considered as potential candidate genes for chilling tolerance. This study will assist in the cloning of the candidate genes responsible for chilling tolerance and molecular breeding of rice for the development of chilling-tolerant rice varieties.

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