Miracle Fruit Leaf Extract: Antioxidant Activity Evaluation, Constituent Identification, and Medical Applications
文献类型： 外文期刊
作者： Liu, Yu-Ge 1 ; Li, Boyan 2 ; Fu, Qiong 1 ; Zhang, Xiu-Mei 1 ; Ma, Fei-Yue 1 ; Hu, Yun 3 ;
作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crop Res Inst, Key Lab Trop Fruit Biol, Minist Agr, Zhanjiang 524091, Peoples R China
2.Guizhou Med Univ, Sch Publ Hlth, Key Lab Environm Pollut Monitoring & Dis Control, Minist Educ,Sch Food Sci, Guiyang, Peoples R China
3.China Tobacco Guizhou Ind Co Ltd, Technol Ctr, Guiyang, Peoples R China
关键词： Antioxidant activity; miracle fruit leaves; paralysis assay; polyphenols; ultra-high performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MSn)
期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）
ISSN： 0003-2719
年卷期：
页码：
收录情况： SCI
摘要： A systematic study was carried out on the antioxidants in miracle fruit leaf extract. The total phenols, total flavonoids, saponins and the antioxidant activities of the extract were evaluated using the 2,2 '-diphenyl-2-picrylhydrazyl (DPPH), 2,2 '-azino-bis-3-ethyl-benzotiazolin-6-sulfonic acid (ABTS), metal chelating capacity (MCC) and ferric reducing antioxidant power (FRAP) assays. The results showed that the leaves contained high phenol concentrations of 90.0 +/- 4.78 mg gallic acid equivalent/g dry weight, total flavonoids at 33.0 +/- 1.92 mg rutin equivalent/g dry weight, and saponins at 27.8 +/- 1.64 mg oleanolic acid equivalent/g dry weight. The extract possessed excellent free radical scavenging ability and high antioxidant capacities. Furthermore, eighteen polyphenol and flavonoid constituents in the extract were identified by using ultra-high performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MSn) method and standard chemicals. The constituents were gallic acid, 5-O-caffeoylquinic acid, 3-O-p-coumaroylquinic acid, 5-O-p-coumaroylquinic acid, 3-O-feruloylquinic acid, 4-O-caffeoylquinic acid, 4-O-p-coumaroylquinic acid dimer, 4-O-p-coumaroylquinic acid, 4-O-feruloylquinic acid, 4-O-feruloylquinic acid, rutin, myricetin-3-O-rhamnoside, quercetin-3-D-galactoside, quercetin-3-glucoside, kaempferol-3-O-glucoside, quercetin-3-rhamnoside, myricetin-O-galloylrhamnoside and quercetin-3-O-(2 ''-galloyl)-rhamnoside.Eight constituents were quantified with values from 0.12 to 4.13 mg center dot g(-1). The extract of miracle fruit leaves was also used in an anti-Alzheimer's disease model for potential medical applications. The assay indicated that the extract provided apparent inhibition for the paralysis of Caenorhabditis elegans at a concentration of 0.02 mg center dot mL(-1). This work demonstrated that miracle fruit leaves are rich in bioactive antioxidants with potential for drug and functional product development.
- 相关文献
作者其他论文 更多>>
-
Ultrasound-assisted extraction of phenolic compounds from macadamia (Macadamia integrifolia) green peel: Purification, identification and antioxidant activities
作者：Zhang, Ming;Li, Ya;Shuai, Xi-xiang;Qiao, Jian;Wei, Chang-bin;Ma, Fei-yue;Zhang, Yu-han;Du, Li-qing;Zhang, Ming;Li, Ya;Shuai, Xi-xiang;Qiao, Jian;Ma, Fei-yue;Zhang, Yu-han;Du, Li-qing;Shuai, Xi-xiang;Du, Li-qing
关键词：Macadamia green peel; Ultrasound-assisted extraction; Purification; Identification; Antioxidant activity
-
Identification of phenolics from miracle berry (Synsepalum dulcificum) leaf extract and its antiangiogenesis and anticancer activities
作者：Ma, Fei-Yue;Zhang, Xiu-Mei;Li, Ya;Zhang, Ming;Tu, Xing-Hao;Du, Li-Qing;Ma, Fei-Yue;Zhang, Xiu-Mei;Li, Ya;Zhang, Ming;Tu, Xing-Hao;Du, Li-Qing;Ma, Fei-Yue;Zhang, Xiu-Mei;Li, Ya;Zhang, Ming;Tu, Xing-Hao;Du, Li-Qing;Ma, Fei-Yue
关键词：miracle berry; phenolics; zebrafish; MCF-7 xenograft; metabolomics
-
Transcriptome Analysis of Watercore in Pineapple
作者：Yao, Yanli;Li, Mingwei;Lin, Wenqiu;Liu, Shenghui;Wu, Qingsong;Fu, Qiong;Zhu, Zhuying;Gao, Yuyao;Zhang, Xiumei;Yao, Yanli;Li, Mingwei;Lin, Wenqiu;Liu, Shenghui;Wu, Qingsong;Fu, Qiong;Zhu, Zhuying;Gao, Yuyao;Zhang, Xiumei;Yao, Yanli
关键词：fruit quality; physiological disorder; gene expression; RNA-seq
-
Preparation of ethylenediamine-modified pectin/alginate/Fe3O4 microsphere and its efficient Pb2+adsorption properties
作者：Li, Ya;Zhang, Ming;Ma, Fei-yue;Qiao, Jian;Chen, Rong-hao;Du, Li-qing;Shuai, Xi-xiang;Chen, Jun;Ma, Fei-yue
关键词：Pectin; Ethylenediamine; Magnetic microsphere; Pb2+; Adsorption
-
Thermostable Cellulases/Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective
作者：Ajeje, Samaila Boyi;Hu, Yun;Song, Guojie;Peter, Sunday Bulus;Afful, Richmond Godwin;Sun, Fubao;Asadollahi, Mohammad Ali;Amiri, Hamid;Abdulkhani, Ali;Sun, Haiyan
关键词：thermostable cellulase; thermostable xylanase; thermophilic microorganism; thermostability; lignocellulose; genetic engineering; enzyme hydrolysis
-
Lonic Liquids-based Ultrasonic -assisted Extraction of Total Flavonoids from Manilkara Zapota Leaves
作者：Ma, Fei-Yue;Fu, Qiong;Zhang, Xiu-Mei;Liu, Yu-Ge;Ma, Fei-Yue;Fu, Qiong;Zhang, Xiu-Mei;Liu, Yu-Ge
关键词：Manilkara zapota leaves;Ionic liquids-based ultrasonic-assisted extraction (ILs-UAE) Total flavonoids;Response surface methodology (RSM)
-
Comparison on the Antioxidant Activities of Extracts from Six Tropical Fruit Tree Leaves
作者：Du, Liqing;Ma, Feiyue;Fu, Qiong;Liu, Yuge;Zhang, Xiumei
关键词：Leaf;Phenolic compounds;Antioxidant activity
