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The accessory protein ORF3 of porcine epidemic diarrhea virus inhibits cellular interleukin-6 and interleukin-8 productions by blocking the nuclear factor-kappa B p65 activation

文献类型： 外文期刊

作者： Wu, Zhijun 1 ; Cheng, Lixin 3 ; Xu, Jiaxin 3 ; Li, Pengfei 5 ; Li, Xingzhi 3 ; Zou, Dehua 3 ; Zhang, Yating 3 ; Wang, Xia 1 ;

作者机构： 1.HeiLongJiang BaYi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Peoples R China

2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150069, Peoples R China

3.HeiLongJiang BaYi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Peoples R China

4.HeiLongJiang BaYi Agr Univ, Biotechnol Ctr, Daqing 163319, Peoples R China

5.Harbin Med Univ, Affiliated Hosp 5, Dept Nephrol, Daqing 163319, Peoples R China

6.HeiLongJiang BaYi Agr Univ, Coll Sci, Daqing 163319, Peoples R China

7.HeiLongJiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar 161005, Peoples R China

关键词： Porcine epidemic diarrhea virus; ORF3 protein; Pro-inflammatory cytokine; NF-kappa B; Innate immune

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2020 年 251 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is an enveloped, single-stranded positive-sense RNA virus that belongs to a porcine entero-pathogenic alphacoronavirus, causing lethal watery diarrhea in piglets. Despite existing study reports the sole accessory protein ORF3 identified as NF-kappa B antagonist, the contribution of PEDV ORF3 to production of the pro-inflammatory cytokines mediated by NF-kappa B signaling remains largely unknown. Thus in this present study, we showed that PEDV ORF3 protein significantly inhibited the productions of pro inflammatory cytokines interleukin-6 (IL-6) and IL-8. The phosphorylation of I kappa B alpha was inhibited by ORF3 protein, but no degradation of I kappa B alpha was induced in ORF3-expressing cells. Furthermore, PEDV ORF3 inhibited NF-kappa B activation through preventing nuclear factor p65 phosphorylation and down-regulating p65 expression level, as well as interfering nuclear translocation of p65, eventually resulting into the inhibition of IL-6 and IL-8 production. Our study definitely links PEDV ORF3 to inhibition of pro-inflammatory cytokines production, which will provide new insight into the molecular mechanisms of NF-kappa B activity inhibited by PEDV proteins to facilitate virus evasion of host innate immune.

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