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Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of Wisteria monocytogenes

文献类型： 外文期刊

作者： Li, Honghuan 1 ; Qiao, Yanjie 1 ; Du, Dongdong 2 ; Wang, Jing 1 ; Ma, Xun 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China

2.Xinjiang Acad Agr & Reclamat Sci, Anal & Testing Ctr, Shihezi 832003, Xinjiang, Peoples R China

3.Xinjiang Prod & Construct Corps, Key Lab Control & Prevent Anim Dis, North 4th Rd, Shihezi 832003, Xinjiang, Peoples R China

关键词： Listeria monocytogenes; oligopeptide permeases; gene deletion; biofilm; virulence

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

ISSN： 1229-845X

年卷期： 2020 年 21 卷 6 期

页码：

收录情况： SCI

摘要： Background: Listeria monocytogenes is a gram-positive bacterium that causes listeriosis mainly in immunocompromised hosts. It can also cause foodborne outbreaks and has the ability to adapt to various environments. Peptide uptake in gram-positive bacteria is enabled by oligopeptide permeases (Opp) in a process that depends on ATP hydrolysis by OppD and F. Previously a putative protein Lmo2193 was predicted to be OppD, but little is known about the role of OppD in major processes of L. monocytogenes, such as growth, virulence, and biofilm formation. Objectives: To determine whether the virulence traits of L. monocytogenes are related to OppD. Methods: In this study, Lmo2193 gene deletion and complementation strains of L. monocytogenes were generated and compared with a wild-type strain for the following: adhesiveness, invasion ability, intracellular survival, proliferation, 50% lethal dose (LD50) to mice, and the amount bacteria in the mouse liver, spleen, and brain. Results: The results showed that virulence of the deletion strain was 1.34 and 0.5 orders of magnitude higher than that of the wild-type and complementation strains, respectively. The function of Lmo2193 was predicted and verified as OppD from the ATPase superfamily. Deletion of Imo2193 affected the normal growth of L. monocylogenes, reduced its virulence in cells and mice, and affected its ability to form biofilms. Conclusions: Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of L. monocytogenes. These effects may be related to OppD's function, which provides a new perspective on the regulation of oligopeptide transporters in L. monocytogenes.

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